Combination flow through injection and isolation valve for high pressure fluids

ABSTRACT

A flow through injection valve having a stationary member, a movable member, a surface of the stationary member interfacing with a surface of the movable member; and at least one pin isolation valve having a flow through internal conduit and movably positioned so that the internal conduit can interface with at least one flow through conduit in the movable member. The pin isolation valves are movably positioned so that the internal conduit is also capable of fluidically communicating with another flow through internal conduit in the movable member. The flow through injection valve can be combined with a similar flow through isolation valve to serve as a multiple valve and typically for replacing a conventional face seal valve of a high pressure liquid chromatography system. The multiple valve allows flow to be transferred through without need for switching or rotating under high pressure. Movement is by rotation or translation.

CROSS REFERENCE RELATED APPLICATION INFORMATION

This application claims priority from U.S. Provisional PatentApplication No. 60/550,930, filed Mar. 5, 2004. The contents of theseapplications are incorporated herein by reference.

BACKGROUND OF THE INVENTION

The invention relates generally to the field of high pressure fluidsand, more specifically, to a combination of multiple isolation valvesthat permit introduction of flow path without interruption of flow fromthe fluid source.

DESCRIPTION OF RELATED ART

Conventional 6-port face shear valves, also referred to as face sealvalves, used in high pressure liquid chromatography (HPLC) provide portsthat interface with the sample, the syringe, the pump, the column andthe two ends of the sample loop. Such face seal valves must be rotatedto switch from one port to another. The rotation of the face seal underhigh pressure inherently causes damage to the plastic mating surfacesbecause the fluid port openings must slide against the rotor surfacecausing fatigue of the rotor material. This results in shortened faceseal valve life. In addition, it is necessary to temporarily block flowduring the sample injection process and sample dispersion occurs.

At higher chromatography pressures, e.g., greater than 15,000 psig or100 MPa, what is needed is a flow-through isolation sample injectionvalve that can provide high sample injection life with minimal sampledistortion and minimal pump pressure pulsing.

BRIEF SUMMARY OF THE INVENTION

To address the above and other issues, the present invention describes acombination of multiple flow-through high pressure isolation valves forhigh pressure fluids, and which is particularly suitable for use in HPLCapplications as a substitute for the conventional face shear valve.

It is an object of this invention to provide a flow-through sampleinjection valve with fluid port openings that do not slide against arotor surface causing fatigue of the rotor material.

It is another object of this invention to provide a flow-through sampleinjection valve which avoids flow of the sample through non-cylindricalpassages so as to minimize sample dispersion.

In a particular aspect of the invention, the present invention isdirected to a flow through injection valve, the flow through injectionvalve comprising:

a stationary member; a movable member, a surface of the stationarymember interfacing with a surface of the movable member; and at leastone pin isolation valve. The at least one pin isolation valve has a flowthrough internal conduit, and is movably disposed so that the internalconduit is capable of fluidically communicating with at least one flowthrough conduit in the movable member, and is movably disposed so thatthe internal conduit is capable of fluidically communicating withanother flow through conduit in the movable member. The movable memberof the flow through injection valve can further comprise first andsecond conduits for interfacing with internal conduits of first andsecond pin isolation valves, with the first and second conduits openingto a surface of the movable member; a third conduit enabling fluidiccommunication between the internal conduits of the first and second pinisolation valves; and a fourth conduit enabling fluidic communicationbetween internal conduits of third and fourth pin isolation valves, thethird pin isolation valve providing fluid flow, the fourth pin isolationvalve exhausting the fluid flow. The movable member can move by rotationaround an axis of rotation or by at least one of linear and curvilineartranslation. One of the at least one pin isolation valves can befluidically coupled to a sample loop of a high pressure liquidchromatography (HPLC) system. One of the at least one pin isolationvalves can be in fluidic communication with a pump supplying highpressure liquid to a high pressure liquid chromatography (HPLC) system.One of the at least one pin isolation valves can be fluidically coupledto a column discharging high pressure liquid from a high pressure liquidchromatography (HPLC) system.

In a specific aspect of the invention, the present invention is directedto a flow through injection valve, the flow through injection valvedisposed around an axis of rotation, the injection valve comprising: atleast two opposing valve ends disposed around the axis of rotation; themovable member comprising a rotor disposed between said valve ends, anaxis of rotation of the rotor being one of parallel and coincident withthe axis of rotation of the injection valve, and the rotor is disposedsuch that orientation of the rotor can change by rotation around theaxis of rotation of the rotor. The rotor has an outer surface; at leasttwo opposing surfaces each intersecting the outer surface; a firstflow-through conduit having an opening on a first of the at least twoopposing surfaces and an opening on a second of the at least twoopposing surfaces; a second flow-through conduit having an opening on afirst of the at least two opposing surfaces and an opening on a secondof the at least two opposing surfaces; a flow through conduit having anopening on the outer surface and an opening on the first of the at leasttwo opposing surfaces; and a flow through conduit having an opening onthe outer surface and an opening on the second of the at least twoopposing surfaces. The rotor further comprises a first sealing annulusfor sealing the openings on the first of the at least two opposingsurfaces; and a second sealing annulus for sealing the openings on thesecond of the at least two opposing surfaces.

The rotor further comprises a first pin isolation valve having aninternal conduit, the first pin isolation valve disposed to moveparallel to the axis of rotation of the injection valve, with the firstpin isolation valve movably disposed so as to be capable of fluidicallycommunicating, through said internal conduit, with the opening on thefirst flow-through channel on the first of the at least two opposingsurfaces, and movably disposed so as to be capable of fluidicallycommunicating, through the internal conduit, with the flow throughconduit having an opening on the outer surface and an opening on thesecond of the at least two opposing surfaces. The rotor furthercomprises a second pin isolation valve having an internal conduit, thesecond pin isolation valve disposed to move parallel to the axis ofrotation of the injection valve, and movably disposed so as to becapable of fluidically communicating, through the internal conduit, withthe opening on the first flow-through channel on the second of the atleast two opposing surfaces, and movably disposed so as to be capable offluidically communicating, through the internal conduit, with the flowthrough conduit having an opening on the outer surface and an opening onthe second of the at least two opposing surfaces.

The rotor further comprises a third pin isolation valve having aninternal conduit, the third pin isolation valve disposed to moveparallel to the centerline of the injection valve, the third pinisolation valve movably disposed so as to be capable of fluidicallycommunicating, through the internal conduit, with the opening on thesecond flow-through channel on the first of the at least two opposingsurfaces, and movably disposed so as to be capable of fluidicallycommunicating, through the internal conduit, with the flow throughconduit having an opening on the outer surface and an opening on thesecond of the at least two opposing surfaces. The rotor furthercomprises a fourth pin isolation valve having an internal conduit, thefourth pin isolation valve disposed to move parallel to the centerlineof the injection valve, the fourth pin isolation valve movably disposedso as to be capable of fluidically communicating, through the internalconduit, with the opening on the second flow-through channel on thesecond of the at least two opposing surfaces, and movably disposed so asto be capable of fluidically communicating, through the internalconduit, with the flow through conduit having an opening on the outersurface and an opening on the second of the at least two opposingsurfaces.

The rotor can further comprise: a rotor clamp having an outer surfaceand an inner surface, the inner surface surrounding at least a portionof the outer surface of the rotor; a first opening on the outer surfaceof the rotor clamp penetrating the rotor clamp to coincide with thefirst opening on the outer surface of the rotor; and a second opening onthe outer surface of the rotor clamp penetrating the rotor clamp tocoincide with the second opening on the outer surface of the rotor. Therotor clamp can further comprise drive means for driving the rotor torotate around the axis of rotation of the rotor. The rotor clamp drivemeans can comprise a gear drive operator or a handle operator.

At least one of the valve ends can comprise: a stator enclosing the atleast one pin isolation valve, the stator adjacent to the rotor; asealing layer enclosed within the stator and enclosing the at least onepin isolation valve for sealing the at least one pin isolation valve; aBelleville spring washer; a Belleville spring; a load washer; and aspherical nut, the Belleville spring washer, the Belleville spring, theload washer and the spherical nut axially arranged to impose an axialforce for sealing the sealing layer enclosing the pin isolation valve.Either of the first and second pin isolation valves can be fluidicallycoupled to a sample loop of a high pressure liquid chromatography (HPLC)system. Either of the third and fourth pin isolation valves can be influidic communication with a pump supplying high pressure liquid to ahigh pressure liquid chromatography (HPLC) system or in fluidiccommunication with a column discharging high pressure liquid to a highpressure liquid chromatography (HPLC) system.

In another embodiment, the present invention is directed to a multiplevalve comprised of: a housing; a rotary flow through isolation valvedisposed within the housing, with the isolation valve oriented in anaxial direction for isolation of fluid flow, the isolation valvedisposed around an axis of rotation, the isolation valve comprising: atleast two opposing valve ends disposed around the axis of rotation; arotor disposed between the valve ends, an axis of rotation of the rotorbeing substantially parallel and coincident with the axis of rotation ofthe isolation valve, with the rotor disposed such that orientation ofthe rotor can change by rotation around the axis of rotation of therotor. The rotor has: an outer surface, at least two opposing surfaceseach intersecting the outer surface; a flow-through conduit having anopening on a first of the at least two opposing surfaces and an openingon a second of the at least two opposing surfaces; a flow throughconduit having an opening on the outer surface and an opening on thefirst of the at least two opposing surfaces; a flow through conduithaving an opening on the outer surface and an opening on the second ofthe at least two opposing surfaces; at least one blank opening on thefirst of the at least two opposing surfaces; and at least one blankopening on the second of the at least two opposing surfaces. The rotorfurther comprises: a first sealing annulus for sealing the openings onthe first of the at least two opposing surfaces, and a second sealingannulus for sealing the openings on the second of the at least twoopposing surfaces. The rotor further comprises: a first pin isolationvalve, the first pin isolation valve disposed to move along the axis ofrotation of the isolation valve, the first pin isolation valve movablydisposed so as to be capable of fluidically communicating with the atleast one blank opening on the first of the at least two opposingsurfaces, and movably disposed so as to be capable of fluidicallycommunicating with the flow through conduit having an opening on theouter surface and an opening on a second of the at least two opposingsurfaces; and a second pin isolation valve, the second pin isolationvalve disposed to move along the centerline of the isolation valve, thesecond pin isolation valve movably disposed so as to be capable offluidically communicating with the at least one blank opening on thesecond of the at least two opposing surfaces, the second pin isolationvalve movably disposed so as to be capable of fluidically communicatingwith the flow through conduit having an opening on the outer surface andan opening on the second of the at least two opposing surfaces. Themultiple valve further comprises: a linear flow through injection valve,the injection valve comprising: a stationary member; a movable member,the stationary member and the movable member interfacing at a surface,the movable member disposed to slide along the surface; a chamberdisposed between the stationary member and the movable member, thechamber bounded by the surface; the movable member having a first flowthrough conduit having a first opening interfacing with the chamber anda second opening on a surface of the movable member not interfacing withthe chamber, the movable member having a second flow through conduithaving a first opening interfacing with the chamber and a second openingon a surface of the movable member not interfacing with the chamber. Themovable member further comprises: a third flow through conduit having afirst opening and a second opening each on a surface of the movablemember interfacing with the chamber; and a fourth flow through conduithaving a first opening and a second opening each on a surface of themovable member interfacing with the chamber.

In yet another embodiment, the present invention is directed to amultiple valve comprised of: a housing; a linear flow through isolationvalve disposed within the housing, the isolation valve comprising: astationary member; a movable member, the stationary member and themovable member interfacing at a surface, the movable member disposed toslide along the surface; a chamber disposed between the stationarymember and the movable member, with the chamber bounded by the surface.The movable member has a first flow through conduit having an openinginterfacing with the chamber and an opening on a surface of the movablemember not interfacing with the chamber, a second flow through conduithaving an opening interfacing with the chamber, and an opening on asurface of the movable member not interfacing with the chamber, a firstblank opening on the surface bounding the chamber, and a second blankopening on the surface bounding the chamber. The multiple valve furthercomprises a linear flow through injection valve, the injection valvecomprising: a stationary member; a movable member, the stationary memberand the movable member interfacing at a surface, the movable memberdisposed to slide along the surface; and a chamber disposed between thestationary member and the movable member, the chamber bounded by thesurface. The movable member has: a first flow through conduit having afirst opening interfacing with the chamber and a second opening on asurface of the movable member not interfacing with the chamber, a secondflow through conduit having a first opening interfacing with the chamberand a second opening on a surface of the movable member not interfacingwith the chamber, a third flow through conduit having a first openingand a second opening each on a surface of the movable member interfacingwith the chamber, and a fourth flow through conduit having a firstopening and a second opening each on a surface of the movable memberinterfacing with the chamber.

The linear flow through injection valve of the multiple valve canfurther comprise: at least one of a (a) first pin isolation valve, (b)second pin isolation valve, (c) third pin isolation valve, and (d)fourth pin isolation valve; the first pin isolation valve having aninternal conduit, the first pin isolation valve disposed within anopening within the stationary member interfacing with the chamber sothat the internal conduit of the first pin isolation valve is movablydisposed to be in fluidic communication with the first opening on afirst flow through conduit of the movable member, and movably disposedto be in fluidic communication with the first opening of the third flowthrough conduit, the second pin isolation valve having an internalconduit, the second pin isolation valve disposed within an openingwithin the stationary member interfacing with the chamber so that theinternal conduit of the second pin isolation valve is movably disposedto be in fluidic communication with the first opening on a second flowthrough conduit of the movable member, and movably disposed to be influidic communication with the second opening of the third flow throughconduit, the third pin isolation valve having an internal conduit, thethird pin isolation valve disposed within an opening within thestationary member interfacing with the chamber so that the internalconduit of the third pin isolation valve is movably disposed to be influidic communication with the first opening of the fourth flow throughconduit, and movably disposed to be in fluidic communication with thefirst opening of the first flow through conduit. The fourth pinisolation valve has an internal conduit, the fourth pin isolation valvedisposed within an opening within the stationary member interfacing withthe chamber so that the internal conduit of the fourth pin isolationvalve is movably disposed to be in fluidic communication with the secondopening of the fourth flow through conduit, and movably disposed to bein fluidic communication with the first opening of the second flowthrough conduit.

Those skilled in the art recognize that any combination such as rotaryisolation and linear injection, linear isolation and rotary injection,rotary injection and rotary isolation, and linear injection and linearisolation multiple valves can be constructed. Furthermore, any of therotary injection, rotary isolation, linear injection, and linearisolation valves can be constructed independently.

The present invention is also directed to a method of operating a flowthrough injection valve, the valve comprising: a movable member, themovable member having first and second conduits for interfacing withinternal conduits of first and second pin isolation valves, the firstand second conduits opening to a surface of the movable member; a thirdconduit enabling fluidic communication between the internal conduits ofthe first and second pin isolation valves; a fourth conduit enablingfluidic communication between internal conduits of third and fourth pinisolation valves, the third pin isolation valve providing fluid flow,the fourth pin isolation valve exhausting the fluid flow;

-   -   (A) wherein the valve is in an initial position of flow        isolation such that the third pin isolation valve providing        fluid flow is in fluidic communication with the fourth pin        isolation valve exhausting the fluid flow, the first pin        isolation valve is in fluidic communication with the first        conduit, and the second pin isolation valve is in fluidic        communication with the second conduit; the method comprises the        steps of: (I) wherein the first pin isolation valve interfaces        with the first conduit, (1) moving the first pin isolation valve        away from the first conduit; (2) moving the movable member, (3)        moving the first pin isolation valve towards the movable member        such that the internal conduit within the first pin isolation        valve interfaces with the third conduit; and (II) wherein the        second pin isolation valve interfaces with the second        conduit, (1) moving the second pin isolation valve away from the        second conduit; (2) moving the movable member, (3) moving the        second pin isolation valve towards the movable member such that        the internal conduit within the second pin isolation valve        interfaces with the third conduit, thereby establishing fluidic        communication between the first and second pin isolation valves;        and        (III) wherein the third pin isolation valve interfaces with the        fourth conduit, (1) moving the third pin isolation valve away        from the fourth conduit; (2) moving the movable member; (3)        moving the third pin isolation valve towards the first conduit        to establish fluidic communication with the internal conduit of        the third pin isolation valve; and (IV) wherein the fourth pin        isolation valve interfaces with the fourth conduit, (1) moving        the fourth pin isolation valve away from the fourth conduit; (2)        moving the movable member; (3) moving the fourth pin isolation        valve towards the second conduit to establish fluidic        communication with the internal conduit of the fourth pin        isolation valve; and (B) wherein the valve is in an initial        position of flow throughput such that at least one of (a) the        third pin isolation valve providing fluid flow interfaces with        the first conduit and (b) the fourth pin isolation valve        exhausting the fluid flow interfaces with the second conduit,        the method comprises the steps of: (III) wherein the third pin        isolation valve interfaces with the first conduit, (1) moving        the third pin isolation valve away from the first conduit, (2)        moving the movable member, and (3) moving the third pin        isolation valve towards the movable member such that the        internal conduit within the third pin isolation valve interfaces        with the fourth conduit; and (IV) wherein the fourth pin        isolation valve interfaces with the second conduit, (1) moving        the fourth pin isolation valve away from the second conduit, (2)        moving the movable member, and (3) moving the fourth pin        isolation valve towards the movable member such that the        internal conduit within the second pin isolation valve        interfaces with the first conduit; and (V) wherein the first pin        isolation valve interfaces with the third conduit, (1) moving        the first pin isolation valve away from said third conduit, (2)        moving the movable member, and (3) moving the first pin        isolation valve towards the movable member such that the        internal conduit within the first pin isolation valve interfaces        with the first conduit; and (VI) wherein the second pin        isolation valve interfaces with the third conduit, (1) moving        the second pin isolation valve away from the third conduit, (2)        moving the movable member, and (3) moving the second pin        isolation valve towards the movable member such that the        internal conduit within the second pin isolation valve        interfaces with the second conduit.

In another embodiment of the present invention, the present invention isdirected also to a method of operating a multiple valve, the multiplevalve comprising a flow through isolation valve, the flow throughisolation valve comprising: a movable member, the movable member havingfirst and second conduits for interfacing with internal conduits offirst and second pin isolation valves, the conduits opening to a surfaceof the movable member; first and second blank openings for interfacingwith the internal conduits of the first and second pin isolation valves,(A) wherein the valve is in an initial position of flow isolation suchthat at least one of (a) the first pin isolation valve providing fluidflow interfaces with the first blank opening and (b) the second pinisolation valve exhausting the fluid flow interfaces with the secondblank opening,

the method comprises the steps of: (I) wherein the first pin isolationvalve interfaces with the first blank opening, (1) moving the first pinisolation valve away from the first blank opening, (2) moving themovable member, and (3) moving the first pin isolation valve towards themovable member such that the internal conduit within the first pinisolation valve interfaces with the first conduit opening to a surfaceof the movable member; and (II) wherein the second pin isolation valveinterfaces with the second blank opening, (1) moving the second pinisolation valve away from the second blank opening,

(2) moving the movable member, and (3) moving the second pin isolationvalve towards the movable member such that the internal conduit withinthe second pin isolation valve interfaces with the second conduitopening to a surface of the movable member, and (B) wherein the valve isin an initial position of flow throughput such that at least one of (a)the first pin isolation valve providing fluid flow interfaces with thefirst conduit and (b) the second pin isolation valve exhausting thefluid flow interfaces with the second conduit, the method comprises thesteps of: (III) wherein the first pin isolation valve interfaces withthe first conduit, (1) moving the first pin isolation valve away fromthe first conduit, (2) moving said movable member, and (3) moving thefirst pin isolation valve towards the movable member such that theinternal conduit within the first pin isolation valve interfaces withthe first blank opening; and (IV) wherein the second pin isolation valveinterfaces with the second conduit, (1) moving the second pin isolationvalve away from the second conduit, (2) moving the movable member, and(3) moving the second pin isolation valve towards the movable membersuch that the internal conduit within the second pin isolation valveinterfaces with the second blank opening.

In the method of operating a flow through injection valve, the first andsecond conduits opening to a surface of the movable member can be influidic communication with a sample loop of a high pressure liquidchromatography (HPLC) system, or the first and second pin isolationvalves can be in fluidic communication with a needle and a syringe of ahigh pressure liquid chromatography (HPLC) system, or the third andfourth pin isolation valves can be in fluidic communication with a pumpand a column of a high pressure liquid chromatography (HPLC) system,

In the method of operating a multiple valve, the multiple valve alsocomprises a flow through injection valve, the flow through injectionvalve comprising: a movable member, the movable member having first andsecond conduits for interfacing with internal conduits of first andsecond pin isolation valves, the first and second conduits opening to asurface of the movable member; a third conduit enabling fluidiccommunication between the internal conduits of the first and second pinisolation valves; a fourth conduit enabling fluidic communicationbetween internal conduits of third and fourth pin isolation valves, thethird pin isolation valve providing fluid flow, the fourth pin isolationvalve exhausting the fluid flow; (A) wherein the valve is in an initialposition of flow isolation such that the third pin isolation valveproviding fluid flow is in fluidic communication with the fourth pinisolation valve exhausting the fluid flow, the first pin isolation valveis in fluidic communication with the first conduit, and the second pinisolation valve is in fluidic communication with the second conduit; themethod comprises the steps of: (I) wherein the first pin isolation valveinterfaces with the first conduit, (1) moving the first pin isolationvalve away from the first conduit; (2) moving the movable member, (3)moving the first pin isolation valve towards the movable member suchthat the internal conduit within the first pin isolation valveinterfaces with the third conduit; and (II) wherein the second pinisolation valve interfaces with the second conduit, (1) moving thesecond pin isolation valve away from the second conduit; (2) moving themovable member, (3) moving the second pin isolation valve towards themovable member such that the internal conduit with the second pinisolation valve interfaces with the third conduit, thereby establishingfluidic communication between the first and second pin isolation valves;and (III) wherein the third pin isolation valve interfaces with saidfourth conduit, (1) moving the third pin isolation valve away from thefourth conduit; (2) moving the movable member; (3) moving the third pinisolation valve towards the first conduit to establish fluidiccommunication with the internal conduit of the third pin isolation valve; and (IV) wherein said fourth pin isolation valve interfaces with thefourth conduit, (1) moving the fourth pin isolation valve away from thefourth conduit; (2) moving the movable member; (3) moving the fourth pinisolation valve towards the second conduit to establish fluidiccommunication with the internal conduit of the fourth pin isolationvalve.

The method of operating a multiple valve also comprises the steps of:(B) wherein the valve is in an initial position of flow throughput suchthat at least one of (a) the third pin isolation valve providing fluidflow interfaces with the first conduit and (b) the fourth pin isolationvalve exhausting the fluid flow interfaces with the second conduit, themethod comprises the steps of: (III) wherein the third pin isolationvalve interfaces with the first conduit, (1) moving the third pinisolation valve away from the first conduit, (2) moving the movablemember, and (3) moving the third pin isolation valve towards the movablemember such that the internal conduit within the third pin isolationvalve interfaces with the fourth conduit; and (IV) wherein the fourthpin isolation valve interfaces with the second conduit, (1) moving thefourth pin isolation valve away from the second conduit, (2) moving themovable member, and (3) moving the fourth pin isolation valve towardsthe movable member such that the internal conduit within the second pinisolation valve interfaces with the first conduit; and (V) wherein thefirst pin isolation valve interfaces with the third conduit, (1) movingthe first pin isolation valve away from the third conduit, (2) movingthe movable member, and (3) moving the first pin isolation valve towardsthe movable member such that the internal conduit within the first pinisolation valve interfaces with the first conduit; and (VI) wherein thesecond pin isolation valve interfaces with the third conduit, (1) movingthe second pin isolation valve away from the third conduit, (2) movingthe movable member, and (3) moving the second pin isolation valvetowards the movable member such that the internal conduit within thesecond pin isolation valve interfaces with the second conduit.

In the method of operating a multiple valve, the first and secondconduits opening to a surface of the movable member of the flow throughinjection valve can be in fluidic communication with a sample loop of ahigh pressure liquid chromatography (HPLC) system, or the first andsecond pin isolation valves of the flow through injection valve are influidic communication with a needle and a syringe of a high pressureliquid chromatography (HPLC) system, or the third and fourth pinisolation valves of the flow through injection valve can be in fluidiccommunication with a pump and a column of a high pressure liquidchromatography (HPLC) system,

BRIEF DESCRIPTION OF THE DRAWINGS

These and other features, benefits and advantages of the presentinvention will become apparent by reference to the following text andfigures, with like reference numbers referring to like structures acrossthe views, wherein:

FIG. 1A illustrates a combination of multiple rotary flow-throughisolation valves of the present invention in a side elevationcross-sectional view in the load position interfacing with a sampleloop.

FIG. 1B illustrates the combination of multiple flow-through rotaryisolation valves of the present invention of FIG. 1A in a transitionposition interfacing with a sample loop.

FIG. 1C illustrates the combination of multiple flow-through rotaryisolation valves of the present invention of FIG. 1A in an injectionposition interfacing with a sample loop.

FIG. 2A is a partial cut-away perspective view of the isolation portionof the combination of the multiple flow-through rotary isolation valvesof FIGS. 1A-1C.

FIG. 2B is a perspective exploded view of the rotor of the isolationportion of the multiple flow-through rotary isolation valve of FIGS.1A-1C.

FIG. 3A is a plan view of the housing of the multiple rotaryflow-through isolation valves of FIGS. 1A-1C.

FIG. 3B is an elevation view of the housing of the multiple flow-throughrotary isolation valves of FIGS. 1A-1C.

FIG. 3C is a detail view of a portion of the rotary isolation valveassembly of FIG. 3A.

FIG. 4A is a separated elevation section view at break lines 4B ofanother embodiment of the present invention as a multiple valvecomprised of a linear isolation valve and a linear injection valve.

FIG. 4B is a separated elevation view at break lines 4A of theembodiment of FIG. 4B.

DETAILED DESCRIPTION OF THE INVENTION

This application incorporates by reference concurrently filed co-pendingprovisional application Ser. No. (Attorney Docket No. WAA-358)

The present invention describes a combination isolation valve ofmultiple flow-through high pressure isolation valves for high pressurefluids. The combination isolation valve can replace the conventionalface shear valve used in HPLC systems. The rotors are designed for usein high pressure fluid systems to permit switching to another flow pathwithout temporarily blocking flow as would occur in face-shear valves asare customarily used in high pressure fluid systems, in particular inhigh pressure liquid chromatography. The sample fluid injection circuitmay be isolated from the remainder of the HPLC system. Each of thecombination multiple flow-through isolation valves include a housinghaving a bore there through and a cylindrical rotor rotatable within thebore.

When the isolate rotor is in its fluid flow position during theinjection phase, fluid flows from a pump, through the isolation valve,to the sample injector circuit, back through another portion of thevalve, and then to a column. By turning the rotor 90°, the fluid stopports prevent the flow of fluid and isolate the sample circuit from theremainder of the HPLC system.

In particular, in FIG. 1A, an inject rotor 11 of an injection valve 300of combination or multiple isolation valve 10 is shown in a load phasein fluidic communication with a needle 12 to a valve pin 1 at a port 14on a side of rotor 11. The combination or multiple isolation valve 10 iscomprised of an isolation valve 200 and the injection valve 300. Samplefluid flows from the pin 1 into internal conduit 16. The sample fluidflows through typically a substantially 90° bend 15 to a port 18 on theouter surface of the rotor 11. The port 18 is preferably fluidicallycoupled to an inlet flexible tube 20 and correspondingly to the sampleloop 22 so that the sample fluid flows into the sample loop 22 and flowsout through an outlet flexible tube 24.

The outlet flexible tube 24 is preferably fluidically coupled to a port26 that is on the outer surface of rotor 11 through a valve pin 2 and inturn to an internal conduit 28. The sample fluid flows through typicallya substantially 90° bend 29 to a port 30 on an opposite side of therotor 11. A syringe 32 can be fluidically coupled to the port 30 so asto provide negative pressure in the flexible tube 24, sample loop 22,flexible tube 20 and needle 12 for drawing up the sample fluid and topermit the sample fluid to be aspirated into the sample loop 22.

The inject rotor 11 includes ports 40 and 42 which interface throughinternal channel 44; and ports 46 and 48 which interface throughinternal channel 50. An annular space 52 is formed on one side of therotor 11 providing fluidic communication between ports 14, 40 and 46. Anannular space 54 is formed on the opposite side of the rotor 11providing fluidic communication between ports 30, 42 and 48.

During the load phase, the inject rotor 11 is isolated from the highpressure pump 101 and column 102 by means of an isolation rotor 61. Thetwo rotors 11 and 61 interface through high pressure tubing 36 and 38.In particular, high pressure tubing 36 is fluidically coupled withinternal channel 50 through valve pin 3 at port 46 while high pressuretubing 38 is fluidically coupled to internal channel 50 through valvepin 4. The high pressure tubing 36 is fluidically coupled to isolaterotor 61 through valve pin 5 which interfaces with the rotor 61 at blankport 82. Correspondingly, the high pressure tubing 38 is fluidicallycoupled to isolation rotor 61 through valve pin 6 which interfaces withthe rotor 61 at blank port 84. Therefore, during the load phase, thepressure within the high pressure tubing 36 and 38 is substantiallyatmospheric, i.e., 0 psig or 0.101 MPa absolute.

The isolation rotor 61 includes ports 62 and 64 which interface throughinternal channel 66. High pressure pump 101 is fluidically coupled toport 70 by means of internal channel 74. The pump 101 interfaces withthe outer surface of rotor 61 at a port 78. Similarly, column 102 isfluidically coupled to port 72 by means of internal channel 76. Thecolumn 102 interfaces with the outer surface of rotor 61 at a port 80.

The inject rotor 11 can include a wash pump interfacing at port 40 and awash discharge interfacing at port 42. Since stagnant flow can occur inthe internal rotor passageway 44, rotor wash supply connection 110 isprovided to connect from a separate wash pump (not shown) while washdischarge connection 112 enables discharge of the used wash solution.

The wash pump washes the internal chamber 44 following sample injection.An annular space 88 is formed on one side of the rotor 61 providingfluidic communication between ports 62 and 70. An annular space 90 isformed on the opposite side of the rotor 61 providing fluidiccommunication between ports 64 and 72.

FIG. 1B illustrates the transition phase between loading of the sampleinto the sample loop 22 and the injection phase where the sample withinthe loop 22 is injected by high pressure pump 101. During the transitionphase, the isolation rotor 61 remains in the same position as during theload phase. Only orientation of the inject rotor 11 is changed.Specifically, the rotor 11 is rotated so that the valve pins 1 and 2 aredisconnected from the sample loop 22, thereby isolating the needle 12and the syringe tube 32 from the sample loop 22. The valve pin 1 isinserted into port 40 while the valve pin 2 is inserted into port 42 sothat the needle and syringe are fluidically coupled to each otherthrough internal conduit 44.

FIG. 1C illustrates the injection phase when high pressure liquid issupplied from the pump 101 to sample loop 22 and on to the column 102.Specifically, in the injection phase, the rotor 11 remains in theposition achieved during the transition phase. The rotor 61 is rotatedso that the valve pins 5 and 6 are disconnected from the blank ports 82and 84, respectively. The valve pin 5 is now connected to port 70 so asto cause fluidic communication between the pump 101 and the highpressure tubing 36. Correspondingly, the valve pin 6 is now connected toport 72 so as to cause fluidic communication between the high pressuretubing 38 and the column 102.

The annular spaces 88 and 90 formed on opposite sides of the rotor 61provide a high pressure seal annulus for the rotor 61.

Those skilled in the art recognize that following the inject phaseillustrated in FIG. 1C, the flow through isolation valve 200 and theflow through injection valve 300 can be returned to the load phase byreversing the operation back to the transition phase illustrated in FIG.1B and subsequently to the load phase illustrated in FIG. 1A.

FIG. 2A illustrates a perspective view of the isolation rotor 61 as itis disposed within a valve body to form a valve assembly 200. Thecomponents of valve assembly 200 typically are centered around an axisof rotation such as centerline 200CL. Specifically, the rotor 61 ispositioned so that stators 202 and 204 are disposed on either end of therotor 61. Belleville springs 220 and 222 deflect the axial loads alongthe centerline 200CL which act on the rotor 61. The Belleville springs220 and 222 and Belleville washers 232 and 234 are mounted on an end ofboth stators 202 and 204 by means of flanges 224 and 226. The loadwashers 224 and 226 are locked into position by spherical nuts 228 and230. Both sets of sealing layers 206 and 208 are compressed by the axialforces imposed by Belleville spring washers 232 and 234, respectively.The rotor 61 is comprised preferably of PEEK (polyetheretherketone) orPEEK blend. The rotor clamp 240 and the stators 202 and 204 arecomprised preferably of Type 316 stainless steel

The foregoing materials are not exclusive and other materials can beapplied by those skilled in the art.

The rotor 61 is shown in a cutaway view disposed between stators 202 and204. The rotor 61 is sealed by a set of three sealing layers 206 and 208set around the valve pins 5 and 6, respectively. The preferred materialsfor the sealing layers 206 and 208 comprise PEEK, PTFE(polytetrafluorethylene), PEEK in that order.

The foregoing materials are not exclusive and other materials can beapplied by those skilled in the art.

The respective ends 242 and 244 of the flow through isolation valve 200can be considered to comprise the stators 202 and 204, the sealinglayers 206 and 208, Belleville spring washers 232 and 234, Bellevillesprings 220 and 222, load washers 224 and 226 and spherical nuts 228 and230.

FIG. 2B is an exploded view of a portion of the components comprising afirst variation of the embodiment of the flow through isolation valve200. Pump supply fitting 101 interfaces with port 78 in the rotor 61 andoutlet supply to column fitting 102 interfaces with port 80 in the rotor61. Face seal valve supply pin 6 is surrounded by stator 204 andinterfaces with one end of rotor 61 while face seal valve discharge pin5 is surrounded by stator 202 and interfaces with the opposite end ofrotor 61. During normal operation, only the pins 5 and 6 which aresurrounded by the stators 202 and 204 are moved either away from or backtowards the rotor 61. The pump supply fitting 101 and outlet supply tocolumn fitting 102 are maintained normally in position except that theyare rotated together with the rotation of the rotor 61. The rotor 61 androtor clamp 94 are rotated around the centerline 200CL by means of drivegear 205.

When the rotor 61 is in its fluid flow position, fluid flows from aseparate high pressure pump, through the isolation valve 200, to thesample injector circuit of injection valve 10, back to the isolationvalve 200, and then to a column.

When the rotor 61 is rotated around axis of rotation centerline 200CL bymeans of drive gear 205 through an angle of preferably 90°, the pins 5and 6 are repositioned to the blank fluid flow stop ports 82 and 84which prevent the flow of fluid and isolate the sample circuit ofinjection valve 10 from the remainder of the HPLC system. Those skilledin the art recognize that the drive gear 205 can be either a separateunit from the rotor clamp 94 or else the drive gear 205 can be anintegral unitary structure combined with the rotor clamp 94 and even therotor 61. In addition, although shown as a drive gear, other means knownto those skilled in the art such as, for example, an operating handlecan be employed.

Although the ports 78 and 80 are preferably offset by an angle of about90° from each other on the outer surface of the rotor, the ports can bealigned to be adjacent to each other. The offset is preferred due to theadvantage of threaded connections for sealing and the resultant need forlarger diameter tap holes. The threaded tap holes are generally 7.9 mm (5/16 in.) in diameter.

FIG. 3A is a plan view of the housing 310 of the multiple flow-throughisolation valves of FIGS. 1A-1C. FIG. 3B is an elevation view of thehousing of the multiple flow-through isolation valves of FIGS. 1A-1C.

The isolation valve assembly 200 is disposed within the housing 310. Theinjection rotor 11 is disposed within injection valve assembly 300around an axis of rotation such as centerline 300CL. The isolation valveassembly 200 and the injection valve assembly 300 are disposed withinthe housing 310 through the end plates 312 and 314 preferably such thatthe axis of rotation centerlines 200CL and 300CL are parallel to eachother. The valve assemblies 200 and 300 are operated by means such as acam mechanism 320 known to those skilled in the art.

FIG. 3C is a detail view of a portion of the isolation valve assembly ofFIG. 3A. As before, the rotor 61 is positioned so that stators 202 and204 are disposed on either end of the rotor 61. Belleville springs 220and 222 deflect the axial loads along the centerline 200CL which act onthe rotor 61. The Belleville spring 220 is mounted on an end of stator202 by means of load washer 224. The load washer 224 is locked intoposition by spherical nut 228. Sealing layer set 206 is compressed bythe axial force imposed by Belleville spring washer 232. The sphericalnuts 228 and 230 (not shown) are supported by, and penetrate through,housing end plates 312 and 314, respectively.

As described previously with respect to FIG. 2A, the rotor 61 is shownin a cutaway view in FIG. 3C disposed between stators 202 and 204. Therotor 61 is sealed by a set of three sealing layers 206 and 208 setaround the pin valves 5 and 6, respectively. As previously noted, thepreferred materials and arrangement for the sealing layers comprisePEEK, PTFE, PEEK in that order. A valve end 240 of the flow throughisolation valve assembly 200 can be considered to comprise the stator202, the sealing layer 206, Belleville spring washer 232, Bellevillespring 220, load washer 224, and spherical nut 228. Those skilled in theart recognize that the opposite valve end of the isolation valveassembly 200 is typically symmetrical and therefore is comprisedtypically of the corresponding symmetrical components.

In addition, those skilled in the art recognize that the isolation valve200 and injection valve 300 of combination isolation valve 10 can alsobe configured by either of the alternate embodiments in any combinationof embodiments described in co-pending U.S. Provisional PatentApplication No. (Docket No. WAA-358), previously disclosed as beingincorporated by reference. That is, either the first and secondembodiments, or the first and third embodiments, or the second and thirdembodiments, or only the second embodiment or only the third embodimentcan be applied correspondingly as isolation valve 200 and injectionvalve 300.

In a second embodiment, FIGS. 4A and 4B illustrate a combination linearflow through isolation valve 800 and linear flow through injection valve850 each of which has a configuration similar to the generallycylindrically shaped second embodiment of rotor 61 illustrated in FIGS.2A and 2B. The linear flow through isolation valve 800 is comprised of astationary member 802 and a movable member 804. The movable member 804is similar to the rotor 202 except that instead of moving in a rotarymotion, the movable member 804 moves by sliding linearly through thestationary member 802. The movable member 804 can have any other type ofcross-section such as, for example but not limited to, an oval shape ora square with smooth rounded corners. The rotary member 804 is madepreferably of either a metal or a polymer or sapphire.

The stationary member 802 is comprised of two surfaces 806 a and 806 bwhich surround the movable slider member 804. The two surfaces 806 a and806 b each include self-energized lip seals 808 a and 808 b. Thestationary member 802 also forms an interfacing surface 810 surroundingthe movable member

Tube fitting 7 from the high pressure pump 101 is inserted into port 352of the movable member 804 where it is sealed in a manner as tosubstantially prevent external leakage. Flow is provided from the highpressure pump 101 to the pin fitting 7 by means of flexible conduit 316and coupling 312. The internal conduit 38 within the fitting 7 is influidic communication with internal conduit 840 within the movablemember 804 and with an open port 860 on the interfacing surface 810. Theopen port 860 is in fluidic communication with a chamber or volume ofspace 812 within the stationary member 802 that is bordered by theinterfacing surface 810. The volume of space 812 within the stationarymember 802 and the movable member 804 are sealed by the self-energizedlip seals 808 a and 808 b. Isolation valve pin 5 penetrates throughstationary member 802 at penetration 822 such that the valve pin 5 canmove linearly up and down.

By means of coupling 62, the internal conduit 58 within valve pin 5 isin fluidic communication with conduit tubing 834 to the pin isolationvalve 3 of linear flow through injection valve 850. Conduit tubing 66from the face seal valve 10 is then fluidically coupled to the internalconduit 82 of pin isolation valve 78 by means of coupling 70.

Similarly, isolation valve pin 6 penetrates through stationary member802 at penetration 824 such that the valve pin 6 can move linearly upand down. The internal conduit 82 within pin isolation valve 78 is thenin fluidic communication with the volume of space 812 within thestationary member 802 that is bordered by the interfacing surface 810.The valve pin 6 is positioned to interface with open port 880 on theinterfacing surface 810. The open port 880 is in fluidic communicationwith the volume of space 812.

To seal the pin isolation valves 5 and 6, the stationary member 802includes self-energized lip seals 820 a and 820 b, respectively. The lipseals are commercially available from Furon, Inc. of Hoosick Falls, N.Y.

During the injection phase, the internal conduit 82 within the pinisolation valve 6 is in fluidic communication also with internal conduit876 within the movable member 804 and with an open port 874 on anopposite end of movable member 804. Fitting 8 is inserted into open port874 so that the internal conduit 96 within fitting 8 is in fluidcommunication with a sample loop 22. The internal conduit 96 within thefitting 8 is in fluidic communication with the column by means offlexible conduit 318 that is coupled to the fitting 8 by coupling 314.

During the load phase, the pin isolation valve 6 is positioned tointerface with blank port 892 on the surface 810 of movable member 804.Similarly, the pin isolation valve 5 is positioned to interface withblank port 888 on the surface 810 of movable member 804. These actionseffectively isolate flow from the high pressure pump to the linear flowthrough injection valve 850 and to the column in the same manner asdiscussed previously for the first embodiment.

A means for moving the moving member 804 laterally is provided such as,but not limited to, a linear motor 864 which is coupled to the movingmember 804 enables the pins 5 and 6 to be shifted between the open ports860 and 880 and the blank ports 888 and 892, respectively.

The linear flow through injection valve 850 is analogous to the rotaryinjection valve 300. The linear valve 850 comprises stationary member802′ and a movable member 804′. The two members 802′ and 804′ interfaceat surface 810′. The movable member 804′ slides along the surface 810′while the stationary member 802′ remains in place. In thisconfiguration, pin isolation valve 3, having an internal conduit 58′ andwhich receives the fluid flow transferred from the high pressure pumpthrough flexible conduit 834, penetrates the stationary member 802′ atport 822′. The pin isolation valve 3 is coupled to conduit 834 by meansof coupling 62′ and is movably disposed within the stationary member802′ such that isolation valve pin 3 can move up and down and so thatthe internal conduit 58 of valve poin 3 is in fluidic communication witha first opening 860′ of a flow through internal conduit 890′ that passesthrough the movable member 804′ to second opening 880′. Both the firstopening 860′ and the second opening 882′ interface with a chamber orvolume of space 812′ within the stationary member 802′ that is borderedby the interfacing surface 810′. The stationary member 802′ and themovable member 804, act to seal the chamber 812′.

Isolation valve pin 4 is movably disposed within the stationary member802′ in second opening 880′ such that isolation valve pin 4 is influidic communication with the second opening 880′ of the enclosed flowthrough channel 890′. Isolation valve pin 4, having an internal conduit96 and which transfers the fluid flow from the high pressure pump 101 tothe column 102 through flexible conduit 836, penetrates the stationarymember 802′ at port 824′ and is coupled to flexible conduit 836 by meansof coupling 854′. The flexible conduit 836 is coupled to the isolationvalve pin 6 of linear isolation valve 800 by means of coupling 70.

Flexible conduit 852′ is fluidically coupled to needle 12 to fluidicallycouple with the internal conduit 812′ of isolation valve pin 1 which ismovably disposed within the stationary member 802′ such that isolationvalve pin 1 can move up and down and so that the internal conduit 812′of valve pin 1 is in fluidic communication with a first opening 892′ ofan enclosed flow through channel 886′ that passes through the movablemember 804′ to fluidically couple to the sample loop 22 through flexibleconduit 834′.

Syringe 32 is fluidically coupled to flexible conduit 856′ which in turnfluidically couples with the internal conduit 858′ of isolation valvepin 2 which is movably disposed within the stationary member 802′ suchthat isolation valve pin 2 can move up and down and so that the internalconduit 858′ of valve pin 2 is in fluidic communication with a firstopening 874′ of a flow through internal conduit 876′ that passes throughthe movable member 804′ to fluidically couple to the sample loop 22through flexible conduit 836′.

Open ports 894′ and 896′ in movable member 804′ serve as ends of flowthrough internal conduit 898′ so that when pin 1 and pin 2 arere-positioned to interface with open ports 894′ and 896′, respectively,the needle 12 and syringe 32 are then fluidically coupled directly toeach other and disconnected from the sample loop 22.

The stationary member 802′ is comprised of two surfaces 806 a′ and 806b′ which surround the movable slider member 804′. The two surfaces 806a′ and 806 b′ each include self-energized lip seals 808 a′ and 808 b′.The stationary member 802′ also forms an interfacing surface 810′surrounding the movable member 804′.

To seal the isolation valve pins 1, 2, 3 and 4, the stationary member802′ includes self-energized lip seals 820 a′ and 820 b′, respectively.

A means for moving the moving member 804′ laterally is provided such as,but not limited to, a linear motor 864′ which is coupled to the movingchamber 804′ enables the pins 1 and 2 to be shifted between the openports 892′ and 874′, and the open ports 894′ and 896′, respectively. Thelinear motor 864′ can be driven by any means known in the art such as byelectrical, hydraulic or pneumatic power.

During the load phase, the isolation valve poin 3 is positioned tointerface with opening 860′ on the surface 810′ of movable member 804′.Similarly, the isolation valve pin 4 is positioned to interface withopening 880′ also on the surface 810′ of movable member 804′. Theseactions effectively isolate flow from the high pressure pump 101 to thesample loop 22 since the flow from the pump 101 is now recirculated fromvalve pin 3 through internal conduit 890′ to the column 102 throughisolation valve pin 4.

The needle 12 is then fluidically coupled to the sample loop 22 by meansof the internal conduit 812′ of isolation valve pin 1 being fluidicallycoupled to the opening 894′ of internal conduit 898′. Correspondingly,the syringe 32 is fluidically coupled to the sample loop 22 by means ofthe internal conduit 898′ being fluidically coupled to opening 896′ ofinternal conduit 876′. The syringe 32 is then used to aspirate thesample fluid into the sample loop 22 from the needle 12.

During the transition phase, the moving member 804′ is shifted laterallyso that isolation valve pin 1 interfaces with opening 894′ and isolationvalve pin 2 interfaces with opening 896′, thereby isolating flow fromthe needle 12 to the syringe 32. Correspondingly, the internal conduit58′ of isolation valve pin 3 interfaces with opening 892′ of internalconduit 886′. Internal conduit 96′ of isolation valve pin 4 interfaceswith opening 874′ of internal conduit 876′.

During the injection phase, as a result of the shifting of the movingmember 804′ during the transition phase, flow from the pump 101 throughisolation valve 800 is then channeled through the sample loop 22 and onto the column 102 through the isolation valve 800.

Another variation of the second embodiment is to design the stationarymember 802 and the moving member 804 as a duplex or mirror-image designso that the moving member 804 further comprises ports and internalconduits for the pump and column, or a second pump and column, to becapable of serving a second face seal valve simultaneously.

Although described with respect to application to high pressure fluids,the various embodiments of the present invention can be applied tofluids at any operating pressure, including sub-atmospheric, i.e.,vacuum applications as well.

The invention has been described herein with reference to particularexemplary embodiments. Certain alterations and modifications may beapparent to those skilled in the art, without departing from the scopeof the invention. The exemplary embodiments are meant to beillustrative, not limiting of the scope of the invention, which isdefined by the appended claims.

1. A flow through injection valve, said flow through injection valvecomprising: a stationary member; a movable member, a surface of saidstationary member interfacing with a surface of said movable member; andat least one pin isolation valve; said at least one pin isolation valvehaving a flow through internal conduit, said at least one pin isolationvalve movably disposed so that said internal conduit is capable offluidically communicating with at least one flow through conduit in saidmovable member, said at least one pin isolation valve movably disposedso that said internal conduit is capable of fluidically communicatingwith another flow through conduit in said movable member.
 2. The flowthrough injection valve according to claim 1 wherein said movable membercomprises: first and second conduits for interfacing with internalconduits of first and second pin isolation valves, said first and secondconduits opening to a surface of said movable member; a third conduitenabling fluidic communication between said internal conduits of saidfirst and second pin isolation valves; and a fourth conduit enablingfluidic communication between internal conduits of third and fourth pinisolation valves, said third pin isolation valve providing fluid flow,said fourth pin isolation valve exhausting said fluid flow.
 3. The flowthrough injection valve according to claim 1, wherein said movablemember moves by rotation around an axis of rotation.
 4. The flow throughinjection valve according to claim 1, wherein said movable member movesby at least one of linear and curvilinear translation.
 5. The flowthrough injection valve according to claim 1, wherein one of said atleast one pin isolation valves is fluidically coupled to a sample loopof a high pressure liquid chromatography (HPLC) system.
 6. The flowthrough injection valve according to claim 1, wherein one of said atleast one pin isolation valves is in fluidic communication with a pumpsupplying high pressure liquid to a high pressure liquid chromatography(HPLC) system.
 7. The flow through injection valve according to claim 1,wherein one of said at least one pin isolation valves is fluidicallycoupled to a column discharging high pressure liquid from a highpressure liquid chromatography (HPLC) system.
 8. A flow throughinjection valve, said injection valve disposed around an axis ofrotation, said injection valve comprising: at least two opposing valveends disposed around the axis of rotation; a movable member comprising arotor disposed between said valve ends, an axis of rotation of saidrotor being one of parallel and coincident with the axis of rotation ofsaid injection valve, said rotor disposed such that orientation of saidrotor can change by rotation around the axis of rotation of said rotor,said rotor having an outer surface; at least two opposing surfaces eachintersecting said outer surface; a first flow-through conduit having anopening on a first of said at least two opposing surfaces and an openingon a second of said at least two opposing surfaces; a secondflow-through conduit having an opening on a first of said at least twoopposing surfaces and an opening on a second of said at least twoopposing surfaces; a flow through conduit having an opening on saidouter surface and an opening on said first of said at least two opposingsurfaces; a flow through conduit having an opening on said outer surfaceand an opening on said second of said at least two opposing surfaces; afirst sealing annulus for sealing said openings on said first of said atleast two opposing surfaces; a second sealing annulus for sealing saidopenings on said second of said at least two opposing surfaces; a firstpin isolation valve having an internal conduit, said first pin isolationvalve disposed to move parallel to the axis of rotation of saidinjection valve, said first pin isolation valve movably disposed so asto be capable of fluidically communicating, through said internalconduit, with said opening on said first flow-through channel on saidfirst of said at least two opposing surfaces, said first pin isolationvalve movably disposed so as to be capable of fluidically communicating,through said internal conduit, with said flow through conduit having anopening on said outer surface and an opening on said second of said atleast two opposing surfaces; a second pin isolation valve having aninternal conduit, said second pin isolation valve disposed to moveparallel to the centerline of said injection valve, said second pinisolation valve movably disposed so as to be capable of fluidicallycommunicating, through said internal conduit, with said opening on saidfirst flow-through channel on said second of said at least two opposingsurfaces, said second pin isolation valve movably disposed so as to becapable of fluidically communicating, through said internal conduit,with said flow through conduit having an opening on said outer surfaceand an opening on said second of said at least two opposing surfaces; athird pin isolation valve having an internal conduit, said third pinisolation valve disposed to move parallel to the centerline of saidinjection valve, said third pin isolation valve movably disposed so asto be capable of fluidically communicating, through said internalconduit, with said opening on said second flow-through channel on saidfirst of said at least two opposing surfaces, said third pin isolationvalve movably disposed so as to be capable of fluidically communicating,through said internal conduit, with said flow through conduit having anopening on said outer surface and an opening on said second of said atleast two opposing surfaces; and a fourth pin isolation valve having aninternal conduit, said fourth pin isolation valve disposed to moveparallel to the centerline of said injection valve, said fourth pinisolation valve movably disposed so as to be capable of fluidicallycommunicating, through said internal conduit, with said opening on saidsecond flow-through channel on said second of said at least two opposingsurfaces, said fourth pin isolation valve movably disposed so as to becapable of fluidically communicating, through said internal conduit,with said flow through conduit having an opening on said outer surfaceand an opening on said second of said at least two opposing surfaces. 9.The flow through injection valve according to claim 8, wherein saidrotor further comprises: a rotor clamp having an outer surface and aninner surface, the inner surface surrounding at least a portion of theouter surface of said rotor; a first opening on the outer surface ofsaid rotor clamp penetrating said rotor clamp to coincide with saidfirst opening on said outer surface of said rotor; and a second openingon the outer surface of said rotor clamp penetrating said rotor clamp tocoincide with said second opening on said outer surface of said rotor.10. The flow through injection valve according to claim 9, wherein saidrotor clamp further comprises drive means for driving said rotor torotate around the axis of rotation of said rotor.
 11. The flow throughinjection valve according to claim 10, wherein said rotor clamp drivemeans comprises a gear drive operator.
 12. The flow through injectionvalve according to claim 10, wherein said rotor clamp drive meanscomprises a handle operator.
 13. The flow through injection valveaccording to claim 8, wherein at least one of said valve ends comprises:a stator enclosing said at least one pin isolation valve, said statoradjacent to said rotor; a sealing layer enclosed within said stator andenclosing said at least one pin isolation valve for sealing said atleast one pin isolation valve; a Belleville spring washer; a Bellevillespring; a load washer; and a spherical nut, said Belleville springwasher, said Belleville spring, said load washer and said spherical nutaxially arranged to impose an axial force for sealing said sealing layerenclosing said pin isolation valve.
 14. The flow through injection valveaccording to claim 13, wherein said sealing layer is comprised of atleast one of PEEK (polyetheretherketone) and PTFE(polytetrafluorethylene)
 15. The flow through injection valve accordingto claim 8, wherein said rotor is comprised of PEEK blend.
 16. The flowthrough injection valve according to claim 9, wherein said rotor clampis comprised of stainless steel.
 17. The flow through injection valveaccording to claim 16, wherein said stainless steel is Type 316stainless steel.
 18. The flow through injection valve according to claim8, wherein either of said first and second pin isolation valves isfluidically coupled to a sample loop of a high pressure liquidchromatography (HPLC) system.
 19. The flow through injection valveaccording to claim 8, wherein either of said third and fourth pinisolation valves is in fluidic communication with a pump supplying highpressure liquid to a high pressure liquid chromatography (HPLC) system.20. The flow through injection valve according to claim 8, whereineither of said third and fourth pin isolation valves is in fluidiccommunication with a column discharging high pressure liquid to a highpressure liquid chromatography (HPLC) system.
 21. A flow throughinjection valve comprising: a stationary member and a movable memberinterfacing at a surface, said movable member disposed to slide alongsaid surface; a chamber disposed between said stationary member and saidmovable member, said chamber bounded by said surface; said movablemember having a first flow through conduit having a first openinginterfacing with said chamber and a second opening on a surface of saidmovable member not interfacing with said chamber, said movable memberhaving a second flow through conduit having a first opening interfacingwith said chamber and a second opening on a surface of said movablemember not interfacing with said chamber, said movable member having athird flow through conduit having a first opening and a second openingeach on a surface of said movable member interfacing with said chamber;and said movable member having a fourth flow through conduit having afirst opening and a second opening each on a surface of said movablemember interfacing with said chamber.
 22. The flow through injectionvalve according to claim 21, further comprising: at least one of a (a)first pin isolation valve, (b) second pin isolation valve, (c) third pinisolation valve, and (d) fourth pin isolation valve; said first pinisolation valve having an internal conduit, said first pin isolationvalve movably disposed within an opening within said stationary memberinterfacing with said chamber so that said internal conduit can be influidic communication with said first opening on said first flow throughconduit of said movable member, said first pin isolation valve movablydisposed so that said internal conduit can be in fluidic communicationwith said first opening of said third conduit within said chamber, saidsecond pin isolation valve having an internal conduit, said second pinisolation valve movably disposed within an opening within saidstationary member interfacing with said chamber so that said internalconduit can be in fluidic communication with said first opening on saidsecond flow through conduit of said movable member, said second pinisolation valve movably disposed so that said internal conduit can be influidic communication with said first opening on said second flowthrough conduit of said movable member, said third pin isolation valvehaving an internal conduit, said third pin isolation valve movablydisposed within an opening within said stationary member interfacingwith said chamber so that said internal conduit can be in fluidiccommunication with said first opening of said fourth flow throughconduit, said third pin isolation valve movably disposed so that saidinternal conduit can be in fluidic communication with said first openingof said first flow through conduit, said fourth pin isolation valvehaving an internal conduit, said fourth pin isolation valve movablydisposed within an opening within said stationary member interfacingwith said chamber so that said internal conduit can be in fluidiccommunication with said second opening of said fourth flow throughconduit, said fourth pin isolation valve movably disposed so that saidinternal conduit can be in fluidic communication with said secondopening of said second flow through conduit.
 23. The flow throughinjection valve according to claim 21, further comprising a housingenclosing said stationary member and said movable member.
 24. The flowthrough injection valve according to claim 22, further comprising ahousing enclosing said stationary member and said movable member and atleast one of said pin isolation valves, said internal conduit of said atleast one pin isolation valve fluidically coupled to a conduitpenetrating said housing.
 25. The flow through injection valve accordingto claim 21, further comprising driving means for driving at least oneof said movable members.
 26. The flow through injection valve accordingto claim 25, wherein said driving means is a linear electric motor. 27.The flow through injection valve according to claim 21, wherein eitherof said first and second pin isolation valves of said flow throughinjection valve is fluidically coupled to a sample loop of a highpressure liquid chromatography (HPLC) system.
 28. The flow throughinjection valve according to claim 21, wherein either of said third andfourth pin isolation valves of said flow through injection valve is influidic communication with a pump supplying high pressure liquid to ahigh pressure liquid chromatography (HPLC) system.
 29. The flow throughinjection valve according to claim 21, wherein either of said third andfourth pin isolation valves of said flow through injection valve is influidic communication with a column discharging high pressure liquidfrom a high pressure liquid chromatography (HPLC) system.
 30. The flowthrough injection valve according to claim 23, wherein said housing ofsaid flow through injection valve is capable of retaining pressuregreater than atmospheric pressure.
 31. The flow through injection valveaccording to claim 4, wherein said movable member is comprised of PEEK(polyetheretherketone) blend.
 32. The flow through injection valveaccording to claim 4, wherein said movable member consists of at leastone of (a) metal, (b) polymer, and (c) sapphire.
 33. The flow throughinjection valve according to claim 4, wherein said interfacing surfacebetween said stationary member and said movable member of said linearinjection valve is sealed by at least one lip seal.
 34. The flow throughinjection valve according to claim 21, wherein at least one of saidopenings of said pin isolation valves is sealed by a lip seal.
 35. Themultiple valve according to claim 33, wherein said lip seal isself-energizing.
 36. The multiple valve according to claim 34, whereinsaid lip seal is self-energizing.
 37. A multiple valve comprised of: ahousing; a rotary flow through isolation valve disposed within saidhousing, said isolation valve oriented in an axial direction forisolation of fluid flow, said isolation valve disposed around acenterline oriented in an axial direction, said isolation valvecomprising: at least two opposing valve ends disposed around thecenterline; a rotor disposed between said valve ends, a centerline ofsaid rotor being substantially parallel and coincident with thecenterline of said isolation valve, said rotor disposed such thatorientation of said rotor can change by rotation around the centerlineof said rotor, said rotor having an outer surface; at least two opposingsurfaces each intersecting said outer surface; a flow-through conduithaving an opening on a first of said at least two opposing surfaces andan opening on a second of said at least two opposing surfaces; a flowthrough conduit having an opening on said outer surface and an openingon said first of said at least two opposing surfaces, a flow throughconduit having an opening on said outer surface and an opening on saidsecond of said at least two opposing surfaces, at least one blankopening on said first of said at least two opposing surfaces, at leastone blank opening on said second of said at least two opposing surfaces,a first sealing annulus for sealing said openings on said first of saidat least two opposing surfaces; and a second sealing annulus for sealingsaid openings on said second of said at least two opposing surfaces afirst pin isolation valve, said first pin isolation valve disposed tomove along the centerline of said isolation valve, said first pinisolation valve movably disposed so as to be capable of fluidicallycommunicating with said at least one blank opening on said first of saidat least two opposing surfaces, said first pin isolation valve movablydisposed so as to be capable of fluidically communicating with said flowthrough conduit having an opening on said outer surface and an openingon a second of said at least two opposing surfaces; a second pinisolation valve, said second pin isolation valve disposed to move alongthe centerline of said isolation valve; said second pin isolation valvemovably disposed so as to be capable of fluidically communicating withsaid at least one blank opening on said second of said at least twoopposing surfaces, said second pin isolation valve movably disposed soas to be capable of fluidically communicating with said flow throughconduit having an opening on said outer surface and an opening on saidsecond of said at least two opposing surfaces; and a rotary flow throughinjection valve disposed within said housing for isolation of the fluidflow to a downstream receptacle, said injection valve disposed around acenterline oriented in an axial direction, said injection valvecomprising: at least two opposing valve ends disposed around thecenterline; a rotor disposed between said valve ends, a centerline ofsaid rotor being one of parallel and coincident with the centerline ofsaid injection valve, said rotor disposed such that orientation of saidrotor can change by rotation around the centerline of said rotor, saidrotor having an outer surface; at least two opposing surfaces eachintersecting said outer surface; a first flow-through conduit having anopening on a first of said at least two opposing surfaces and an openingon a second of said at least two opposing surfaces; a secondflow-through conduit having an opening on a first of said at least twoopposing surfaces and an opening on a second of said at least twoopposing surfaces; a flow through conduit having an opening on saidouter surface and an opening on said first of said at least two opposingsurfaces; a flow through conduit having an opening on said outer surfaceand an opening on said second of said at least two opposing surfaces; afirst sealing annulus for sealing said openings on said first of said atleast two opposing surfaces; a second sealing annulus for sealing saidopenings on said second of said at least two opposing surfaces; a firstpin isolation valve having an internal conduit, said first pin isolationvalve disposed to move parallel to the centerline of said injectionvalve, said first pin isolation valve movably disposed so as to becapable of fluidically communicating with said opening on said firstflow-through channel on said first of said at least two opposingsurfaces, said first pin isolation valve movably disposed so as to becapable of fluidically communicating with said flow through conduithaving an opening on said outer surface and an opening on said second ofsaid at least two opposing surfaces; a second pin isolation valve havingan internal conduit, said second pin isolation valve disposed to moveparallel to the centerline of said injection valve, said second pinisolation valve movably disposed so as to be capable of fluidicallycommunicating with said opening on said first flow-through channel onsaid second of said at least two opposing surfaces, said second pinisolation valve movably disposed so as to be capable of fluidicallycommunicating with said flow through conduit having an opening on saidouter surface and an opening on said second of said at least twoopposing surfaces; a third pin isolation valve having an internalconduit, said third pin isolation valve disposed to move parallel to thecenterline of said injection valve, said third pin isolation valvemovably disposed so as to be capable of fluidically communicating withsaid opening on said second flow-through channel on said first of saidat least two opposing surfaces, said third pin isolation valve movablydisposed so as to be capable of fluidically communicating with said flowthrough conduit having an opening on said outer surface and an openingon said second of said at least two opposing surfaces; and a fourth pinisolation valve having an internal conduit, said fourth pin isolationvalve disposed to move parallel to the centerline of said injectionvalve said fourth pin isolation valve movably disposed so as to becapable of fluidically communicating with said opening on said secondflow-through channel on said second of said at least two opposingsurfaces, said fourth pin isolation valve movably disposed so as to becapable of fluidically communicating with said flow through conduithaving an opening on said outer surface and an opening on said second ofsaid at least two opposing surfaces.
 38. The multiple valve according toclaim 37, wherein said first pin isolation valve of said rotary flowthrough isolation valve is fluidically coupled to said third pinisolation valve of said rotary flow through injection valve.
 39. Themultiple valve according to claim 37, wherein said second pin isolationvalve of said rotary flow through isolation valve is fluidically coupledto said fourth pin isolation valve of said rotary flow through injectionvalve.
 40. The multiple valve according to claim 37, wherein either ofsaid rotors further comprises: a rotor clamp having an outer surface andan inner surface, the inner surface surrounding at least a portion ofthe outer surface of said rotor, a first opening on the outer surface ofsaid rotor clamp penetrating said rotor clamp to coincide with saidfirst opening on said outer surface of said rotor, and a second openingon the outer surface of said rotor clamp penetrating said rotor clamp tocoincide with said second opening on said outer surface of said rotor.41. The multiple valve according to claim 40, wherein said rotary flowthrough isolation valve further comprises at least one of a (a) thirdpin isolation valve, and (b) fourth pin isolation valve; said third pinisolation valve having an internal conduit, said rotary flow throughthird pin isolation valve disposed within said first opening on saidouter surface of said rotor clamp so that said internal conduit of saidthird pin isolation valve is disposed to be in fluidic communicationwith said opening on said outer surface of said flow through conduithaving an opening on said outer surface and an opening on said first ofsaid at least two surfaces intersecting said outer surface of saidrotor, said fourth pin isolation valve having an internal conduit, saidfourth pin isolation valve disposed within said second opening on saidouter surface of said rotor clamp so that said internal conduit of saidfourth pin isolation valve is disposed to be in fluidic communicationwith said opening on said outer surface of said flow through conduithaving an opening on said outer surface and an opening on said second ofsaid at least two surfaces intersecting said outer surface of saidrotor.
 42. The multiple valve according to claim 40, wherein said rotorclamp further comprises drive means for driving said rotor to rotatearound the centerline of said rotor.
 43. The multiple valve according toclaim 42, wherein said rotor clamp drive means comprises a gear driveoperator.
 44. The multiple valve according to claim 42, wherein saidrotor clamp drive means comprises a handle operator.
 45. The multiplevalve according to claim 37, wherein at least one of said valve endscomprises: a stator enclosing said at least one pin isolation valve,said stator adjacent to said rotor; a sealing layer enclosed within saidstator and enclosing said at least one pin isolation valve for sealingsaid at least one pin isolation valve; a Belleville spring washer; aBelleville spring; a load washer; and a spherical nut, said Bellevillespring washer, said Belleville spring, said load washer and saidspherical nut axially arranged to impose an axial force for sealing saidsealing layer enclosing said pin isolation valve.
 46. The multiple valveaccording to claim 45, wherein said sealing layer is comprised of atleast one of PEEK (polyetheretherketone) and PTFE(polytetrafluorethylene)
 47. The multiple valve according to claim 37,wherein at least one of said rotors is comprised of PEEK blend.
 48. Themultiple valve according to claim 40, wherein said rotor clamp iscomprised of stainless steel.
 49. The multiple valve according to claim48, wherein said stainless steel is Type 316 stainless steel.
 50. Themultiple valve according to claim 37, wherein either of said first andsecond pin isolation valves of said rotary flow through injection valveis fluidically coupled to a sample loop of a high pressure liquidchromatography (HPLC) system.
 51. The multiple valve according to claim41, wherein either of said third and fourth pin isolation valves of saidrotary flow through isolation valve is fluidically coupled to a pumpsupplying high pressure liquid to a high pressure liquid chromatography(HPLC) system.
 52. The multiple valve according to claim 41, whereineither of said third and fourth pin isolation valves of said rotary flowthrough isolation valve is fluidically coupled to a column discharginghigh pressure liquid to a high pressure liquid chromatography (HPLC)system.
 53. A multiple valve comprised of: a housing; a rotary flowthrough isolation valve disposed within said housing, said isolationvalve oriented in an axial direction for isolation of fluid flow, saidisolation valve disposed around an axis of rotation, said isolationvalve comprising: at least two opposing valve ends disposed around theaxis of rotation; a rotor disposed between said valve ends, an axis ofrotation of said rotor being substantially parallel and coincident withthe axis of rotation of said isolation valve, said rotor disposed suchthat orientation of said rotor can change by rotation around the axis ofrotation of said rotor, said rotor having an outer surface, at least twoopposing surfaces each intersecting said outer surface; a flow-throughconduit having an opening on a first of said at least two opposingsurfaces and an opening on a second of said at least two opposingsurfaces; a flow through conduit having an opening on said outer surfaceand an opening on said first of said at least two opposing surfaces; aflow through conduit having an opening on said outer surface and anopening on said second of said at least two opposing surfaces, at leastone blank opening on said first of said at least two opposing surfaces;at least one blank opening on said second of said at least two opposingsurfaces; a first sealing annulus for sealing said openings on saidfirst of said at least two opposing surfaces, and a second sealingannulus for sealing said openings on said second of said at least twoopposing surfaces; a first pin isolation valve, said first pin isolationvalve disposed to move along the centerline of said isolation valve,said first pin isolation valve movably disposed so as to be capable offluidically communicating with said at least one blank opening on saidfirst of said at least two opposing surfaces, said first pin isolationvalve movably disposed so as to be capable of fluidically communicatingwith said flow through conduit having an opening on said outer surfaceand an opening on a second of said at least two opposing surfaces; asecond pin isolation valve, said second pin isolation valve disposed tomove along the centerline of said isolation valve, said second pinisolation valve movably disposed so as to be capable of fluidicallycommunicating with said at least one blank opening on said second ofsaid at least two opposing surfaces, said second pin isolation valvemovably disposed so as to be capable of fluidically communicating withsaid flow through conduit having an opening on said outer surface and anopening on said second of said at least two opposing surfaces; and alinear flow through injection valve, said injection valve comprising: astationary member; a movable member; said stationary member and saidmovable member interfacing at a surface, said movable member disposed toslide along said surface; a chamber disposed between said stationarymember and said movable member, said chamber bounded by said surface;said movable member having a first flow through conduit having a firstopening interfacing with said chamber and a second opening on a surfaceof said movable member not interfacing with said chamber, said movablemember having a second flow through conduit having a first openinginterfacing with said chamber and a second opening on a surface of saidmovable member not interfacing with said chamber, said movable memberhaving a third flow through conduit having a first opening and a secondopening each on a surface of said movable member interfacing with saidchamber; said movable member having a fourth flow through conduit havinga first opening and a second opening each on a surface of said movablemember interfacing with said chamber; and a second blank opening on saidsurface bounding said chamber.
 54. The multiple valve according to claim53, wherein said linear flow through injection valve further comprises:at least one of a (a) first pin isolation valve, (b) second pinisolation valve, (c) third pin isolation valve, and (d) fourth pinisolation valve; said first pin isolation valve having an internalconduit, said first pin isolation valve disposed within an openingwithin said stationary member interfacing with said chamber so that saidinternal conduit of said first pin isolation valve is movably disposedto be in fluidic communication with said first opening on a first flowthrough conduit of said movable member, said internal conduit of saidfirst pin isolation valve movably disposed to be in fluidiccommunication with said first opening of said third fluid flow throughconduit, said second pin isolation valve having an internal conduit,said second pin isolation valve disposed within an opening within saidstationary member interfacing with said chamber so that said internalconduit of said second pin isolation valve is movably disposed to be influidic communication with said first opening on a second flow throughconduit of said movable member, said internal conduit of said second pinisolation valve movably disposed to be in fluidic communication withsaid second opening of said third flow through conduit, said third pinisolation valve having an internal conduit, said third pin isolationvalve disposed within an opening within said stationary memberinterfacing with said chamber so that said internal conduit of saidthird pin isolation valve is movably disposed to be in fluidiccommunication with said first opening of said fourth flow throughconduit, said internal conduit of said third pin isolation valve movablydisposed to be in fluidic communication with said first opening of saidfirst flow through conduit, said fourth pin isolation valve having aninternal conduit, said fourth pin isolation valve disposed within anopening within said stationary member interfacing with said chamber sothat said internal conduit of said fourth pin isolation valve is movablydisposed to be in fluidic communication with said second opening of saidfourth flow through conduit, said internal conduit of said fourth pinisolation valve movably disposed to be in fluidic communication withsaid first opening on said second flow through conduit.
 55. The multiplevalve according to claim 53, wherein said linear flow through injectionvalve further comprises a housing enclosing said stationary member andsaid movable member.
 56. The multiple valve according to claim 54,wherein said linear flow through injection valve further comprises ahousing enclosing said stationary member and said movable member and atleast one of said pin isolation valves, said internal conduit of said atleast one pin isolation valve fluidically coupled to a conduitpenetrating said housing.
 57. The multiple valve according to claim 53,wherein said linear injection valve further comprises drive means formoving said movable member.
 58. The multiple valve according to claim57, wherein said drive means is a linear motor.
 59. The multiple valveaccording to claim 54, wherein either of said first and second pinisolation valves of said linear injection valve is fluidically coupledto a sample loop of a high pressure liquid chromatography (HPLC) system.60. The multiple valve according to claim 54, wherein either of saidthird and fourth pin isolation valves of said rotary isolation valve isfluidically coupled to a pump supplying high pressure liquid to a highpressure liquid chromatography (HPLC) system.
 61. The multiple valveaccording to claim 54, wherein either of said third and fourth pinisolation valves of said rotary isolation valve is fluidically coupledto a column discharging high pressure liquid from a high pressure liquidchromatography (HPLC) system.
 62. The multiple valve according to claim55, wherein said housing of said linear injection valve is capable ofretaining pressure greater than atmospheric pressure.
 63. The multiplevalve according to claim 56, wherein said housing of said linearinjection valve is capable of retaining pressure greater thanatmospheric pressure.
 64. The multiple valve according to claim 53,wherein said movable member is comprised of PEEK (polyetheretherketone)blend.
 65. The multiple valve according to claim 53, wherein said firstpin isolation valve of said flow through isolation valve is fluidicallycoupled to said third pin isolation valve of said flow through injectionvalve.
 66. The multiple valve according to claim 53, wherein said secondpin isolation valve of said rotary flow through isolation valve isfluidically coupled to said fourth pin isolation valve of said rotaryflow through injection valve.
 67. The multiple valve according to claim53, wherein said rotor further comprises a rotor clamp having an outersurface and an inner surface, the inner surface surrounding at least aportion of the outer surface of said rotor, a first opening on the outersurface of said rotor clamp penetrating said rotor clamp to coincidewith said first opening on said outer surface of said rotor, and asecond opening on the outer surface of said rotor clamp penetrating saidrotor clamp to coincide with said second opening on said outer surfaceof said rotor.
 68. The multiple valve according to claim 67, whereinsaid rotary flow through isolation valve further comprises at least oneof a (a) third pin isolation valve, and (b) fourth pin isolation valve;said third pin isolation valve having an internal conduit, said thirdpin isolation valve disposed within said first opening on said outersurface of said rotor clamp so that said internal conduit of said thirdpin isolation valve is disposed to be in fluidic communication with saidopening on said outer surface of said flow through conduit having anopening on said outer surface and an opening on said first of said atleast two surfaces intersecting said outer surface of said rotor, saidfourth pin isolation valve having an internal conduit, said fourth pinisolation valve disposed within said second opening on said outersurface of said rotor clamp so that said internal conduit of said fourthpin isolation valve is disposed to be in fluidic communication with saidopening on said outer surface of said flow through conduit having anopening on said outer surface and an opening on said second of said atleast two surfaces intersecting said outer surface of said rotor. 69.The multiple valve according to claim 68, wherein at least one of saidthird and fourth pin isolation valves of said rotary flow throughisolation valve is disposed within one of said openings on said outersurface of said rotor clamp by means of a threaded compressionconnection.
 70. The multiple valve according to claim 67, wherein saidrotor clamp further comprises drive means for driving said rotor torotate around the axis of rotation of said rotor.
 71. The multiple valveaccording to claim 70, wherein said rotor clamp drive means comprises agear drive operator.
 72. The multiple valve according to claim 70,wherein said rotor clamp drive means comprises a handle operator. 73.The multiple valve according to claim 53, wherein said interfacingsurface between said stationary member and said movable member of saidlinear injection valve is sealed by at least one lip seal.
 74. Themultiple valve according to claim 54, wherein at least one of saidopenings of said pin isolation valves of said linear injection valve issealed by a lip seal.
 75. The multiple valve according to claim 73wherein said lip seal is self-energizing.
 76. The multiple valveaccording to claim 74, wherein said lip seal is self-energizing.
 77. Theflow through isolation valve according to claim 53, wherein said movablemember consists of at least one of (a) metal, (b) polymer, and (c)sapphire.
 78. The multiple valve according to claim 53, wherein at leastone of said valve ends comprises: a stator enclosing said at least onepin isolation valve, said stator adjacent to said rotor; a sealing layerenclosed within said stator and enclosing said at least one pinisolation valve for sealing said at least one pin isolation valve; aBelleville spring washer; a Belleville spring; a load washer; and aspherical nut, said Belleville spring washer, said Belleville spring,said load washer and said spherical nut axially arranged to impose anaxial force for sealing said sealing layer enclosing said pin isolationvalve.
 79. The multiple valve according to claim 78, wherein saidsealing layer is comprised of at least one of PEEK(polyetheretherketone) and PTFE (polytetrafluorethylene)
 80. Themultiple valve according to claim 53, wherein said rotor is comprised ofPEEK blend.
 81. The multiple valve according to claim 67, wherein saidrotor clamp is comprised of stainless steel.
 82. The multiple valveaccording to claim 81, wherein said stainless steel is ASTM Type 316stainless steel.
 83. A multiple valve comprised of: a housing; a linearflow through isolation valve disposed within said housing, saidisolation valve comprising a stationary member; a movable member; saidstationary member and said movable member interfacing at a surface, saidmovable member disposed to slide along said surface; a chamber disposedbetween said stationary member and said movable member, said chamberbounded by said surface; said movable member having a first flow throughconduit having an opening interfacing with said chamber and an openingon a surface of said movable member not interfacing with said chamber,said movable member having a second flow through conduit having anopening interfacing with said chamber and an opening on a surface ofsaid movable member not interfacing with said chamber, a first blankopening on said surface bounding said chamber, and a second blankopening on said surface bounding said chamber; and a rotary flow throughinjection valve disposed within said housing for isolation of the fluidflow to a downstream receptacle, said injection valve disposed around aan axis of rotation oriented in said injection valve comprising: atleast two opposing valve ends disposed around the centerline; a rotordisposed between said valve ends, a centerline of said rotor being oneof parallel and coincident with the centerline of said injection valve,said rotor disposed such that orientation of said rotor can change byrotation around the centerline of said rotor, said rotor having an outersurface, at least two opposing surfaces each intersecting said outersurface, a first flow-through conduit having an opening on a first ofsaid at least two opposing surfaces and an opening on a second of saidat least two opposing surfaces; a second flow-through conduit having anopening on a first of said at least two opposing surfaces and an openingon a second of said at least two opposing surfaces; a flow throughconduit having an opening on said outer surface and an opening on saidfirst of said at least two opposing surfaces; a flow through conduithaving an opening on said outer surface and an opening on said second ofsaid at least two opposing surfaces; a first sealing annulus for sealingsaid openings on said first of said at least two opposing surfaces; asecond sealing annulus for sealing said openings on said second of saidat least two opposing surfaces; a first pin isolation valve, said firstpin isolation valve disposed to move parallel to the centerline of saidinjection valve, said first pin isolation valve movably disposed so asto be capable of fluidically communicating with said opening on saidfirst flow-through channel on said first of said at least two opposingsurfaces, said first pin isolation valve movably disposed so as to becapable of fluidically communicating with said flow through conduithaving an opening on said outer surface and an opening on said second ofsaid at least two opposing surfaces; a second pin isolation valve, saidsecond pin isolation valve disposed to move parallel to the centerlineof said injection valve, said second pin isolation valve movablydisposed so as to be capable of fluidically communicating with saidopening on said first flow-through channel on said second of said atleast two opposing surfaces; said second pin isolation valve movablydisposed so as to be capable of fluidically communicating with said flowthrough conduit having an opening on said outer surface and an openingon said second of said at least two opposing surfaces; a third pinisolation valve, said third pin isolation valve disposed to moveparallel to the centerline of said injection valve, said third pinisolation valve movably disposed so as to be capable of fluidicallycommunicating with said opening on said second flow-through channel onsaid first of said at least two opposing surfaces, said third pinisolation valve movably disposed so as to be capable of fluidicallycommunicating with said flow through conduit having an opening on saidouter surface and an opening on said second of said at least twoopposing surfaces; and a fourth pin isolation valve, said fourth pinisolation valve disposed to move parallel to the centerline of saidinjection valve, said fourth pin isolation valve movably disposed so asto be capable of fluidically communicating with said opening on saidsecond flow-through channel on said second of said at least two opposingsurfaces, said fourth pin isolation valve movably disposed so as to becapable of fluidically communicating with said flow through conduithaving an opening on said outer surface and an opening on said second ofsaid at least two opposing surfaces.
 84. The multiple valve according toclaim 83, wherein said linear flow through isolation valve furthercomprises: at least one of a (a) first pin isolation valve, (b) secondpin isolation valve, (c) third pin isolation valve, and (d) fourth pinisolation valve; said first pin isolation valve having an internalconduit, said first pin isolation valve disposed within an openingwithin said stationary member interfacing with said chamber so that saidinternal conduit of said first pin isolation valve is movably disposedto be in fluidic communication with said first opening on a first flowthrough conduit of said movable member, said internal conduit of saidfirst pin isolation valve movably disposed to be in fluidiccommunication with said first blank opening on said surface boundingsaid chamber, said second pin isolation valve having an internalconduit, said second pin isolation valve disposed within an openingwithin said stationary member interfacing with said chamber so that saidinternal conduit of said second pin isolation valve is movably disposedto be in fluidic communication with said first opening on a second flowthrough conduit of said movable member, said internal conduit of saidsecond pin isolation valve movably disposed to be in fluidiccommunication with said second blank opening on said surface boundingsaid chamber, said third pin isolation valve having an internal conduit,said third pin isolation valve disposed within an opening within saidstationary member interfacing with said chamber so that said internalconduit of said third pin isolation valve is movably disposed to be influidic communication with said first opening of said third flow throughconduit, said internal conduit of said third pin isolation valve movablydisposed to be in fluidic communication with said first blank opening onsaid surface bounding said chamber, said fourth pin isolation valvehaving an internal conduit, said fourth pin isolation valve disposedwithin an opening within said stationary member interfacing with saidchamber so that said internal conduit of said fourth pin isolation valveis movably disposed to be in fluidic communication with said secondopening of said third flow through conduit, said internal conduit ofsaid fourth pin isolation valve movably disposed to be in fluidiccommunication with said second blank opening on said surface boundingsaid chamber.
 85. The multiple valve according to claim 83, wherein saidlinear flow through isolation valve further comprises a housingenclosing said stationary member and said movable member.
 86. Themultiple valve according to claim 84, wherein said linear flow throughisolation valve further comprises a housing enclosing said stationarymember and said movable member and at least one of said pin isolationvalves, said internal conduit of said at least one pin isolation valvefluidically coupled to a conduit penetrating said housing.
 87. Themultiple valve according to claim 83, wherein said linear isolationvalve further comprises drive means for moving said movable member. 88.The multiple valve according to claim 87, wherein said drive means is alinear motor.
 89. The multiple valve according to claim 84, whereineither of said first and second pin isolation valves of said rotaryinjection valve is fluidically coupled to a sample loop of a highpressure liquid chromatography (HPLC) system.
 90. The multiple valveaccording to claim 84, wherein either of said third and fourth pinisolation valves of said linear isolation valve is fluidically coupledto a pump supplying high pressure liquid to a high pressure liquidchromatography (HPLC) system.
 91. The multiple valve according to claim84, wherein either of said third and fourth pin isolation valves of saidlinear isolation valve is fluidically coupled to a column discharginghigh pressure liquid from a high pressure liquid chromatography (HPLC)system.
 92. The multiple valve according to claim 85, wherein saidhousing of said linear isolation valve is capable of retaining pressuregreater than atmospheric pressure.
 93. The multiple valve according toclaim 86, wherein said housing of said linear isolation valve is capableof retaining pressure greater than atmospheric pressure.
 94. Themultiple valve according to claim 83, wherein said movable member iscomprised of PEEK (polyetheretherketone) blend.
 95. The multiple valveaccording to claim 83, wherein said first pin isolation valve of saidlinear isolation valve is fluidically coupled to said third pinisolation valve of said rotary injection valve.
 96. The multiple valveaccording to claim 83, wherein said second pin isolation valve of saidlinear isolation valve is fluidically coupled to said fourth pinisolation valve of said rotary injection valve.
 97. The multiple valveaccording to claim 83, wherein said rotor further comprises a rotorclamp having an outer surface and an inner surface, the inner surfacesurrounding at least a portion of the outer surface of said rotor, afirst opening on the outer surface of said rotor clamp penetrating saidrotor clamp to coincide with said first opening on said outer surface ofsaid rotor, and a second opening on the outer surface of said rotorclamp penetrating said rotor clamp to coincide with said second openingon said outer surface of said rotor.
 98. The multiple valve according toclaim 97, wherein said rotary flow through injection valve furthercomprises at least one of a (a) third pin isolation valve, and (b)fourth pin isolation valve; said third pin isolation valve having aninternal conduit, said third pin isolation valve disposed within saidfirst opening on said outer surface of said rotor clamp so that saidinternal conduit of said third pin isolation valve is disposed to be influidic communication with said opening on said outer surface of saidflow through conduit having an opening on said outer surface and anopening on said first of said at least two surfaces intersecting saidouter surface of said rotor, said fourth pin isolation valve having aninternal conduit, said fourth pin isolation valve disposed within saidsecond opening on said outer surface of said rotor clamp so that saidinternal conduit of said fourth pin isolation valve is disposed to be influidic communication with said opening on said outer surface of saidflow through conduit having an opening on said outer surface and anopening on said second of said at least two surfaces intersecting saidouter surface of said rotor.
 99. The multiple valve according to claim98, wherein at least one of said third and fourth pin isolation valvesis disposed within one of said openings on said outer surface of saidrotor clamp by means of a threaded compression connection.
 100. Themultiple valve according to claim 97, wherein said rotor clamp furthercomprises drive means for driving said rotor to rotate around thecenterline of said rotor.
 101. The multiple valve according to claim100, wherein said rotor clamp drive means comprises a gear driveoperator.
 102. The multiple valve according to claim 100, wherein saidrotor clamp drive means comprises a handle operator.
 103. The multiplevalve according to claim 83, wherein at least one of said valve endscomprises: a stator enclosing said at least one pin isolation valve,said stator adjacent to said rotor; a sealing layer enclosed within saidstator and enclosing said at least one pin isolation valve for sealingsaid at least one pin isolation valve; a Belleville spring washer; aBelleville spring; a load washer; and a spherical nut, said Bellevillespring washer, said Belleville spring, said load washer and saidspherical nut axially arranged to impose an axial force for sealing saidsealing layer enclosing said pin isolation valve.
 104. The multiplevalve according to claim 103, wherein said sealing layer is comprised ofat least one of PEEK (polyetheretherketone) and PTFE(polytetrafluorethylene)
 105. The multiple valve according to claim 83,wherein said rotor is comprised of PEEK blend.
 106. The multiple valveaccording to claim 97, wherein said rotor clamp is comprised ofstainless steel.
 107. The multiple valve according to claim 106, whereinsaid stainless steel is ASTM Type 316 stainless steel.
 108. The multiplevalve according to claim 83, wherein said interfacing surface betweensaid stationary member and said movable member of said linear isolationvalve is sealed by at least one lip seal.
 109. The multiple valveaccording to claim 84, wherein at least one of said openings of said pinisolation valves of said linear isolation valve is sealed by a lip seal.110. The multiple valve according to claim 108 wherein said lip seal isself-energizing.
 111. The multiple valve according to claim 109, whereinsaid lip seal is self-energizing.
 112. The flow through isolation valveaccording to claim 56, wherein said movable member consists of at leastone of (a) metal, (b) polymer, and (c) sapphire.
 113. A multiple valvecomprised of: a housing; a linear flow through isolation valve disposedwithin said housing, said isolation valve comprising: a stationarymember; a movable member, said stationary member and said movable memberinterfacing at a surface, said movable member disposed to slide alongsaid surface; a chamber disposed between said stationary member and saidmovable member, said chamber bounded by said surface; said movablemember having a first flow through conduit having an opening interfacingwith said chamber and an opening on a surface of said movable member notinterfacing with said chamber, said movable member having a second flowthrough conduit having an opening interfacing with said chamber and anopening on a surface of said movable member not interfacing with saidchamber, a first blank opening on said surface bounding said chamber,and a second blank opening on said surface bounding said chamber; and alinear flow through injection valve, said injection valve comprising astationary member; a movable member, said stationary member and saidmovable member interfacing at a surface, said movable member disposed toslide along said surface; a chamber disposed between said stationarymember and said movable member, said chamber bounded by said surface;said movable member having a first flow through conduit having a firstopening interfacing with said chamber and a second opening on a surfaceof said movable member not interfacing with said chamber, said movablemember having a second flow through conduit having a first openinginterfacing with said chamber and a second opening on a surface of saidmovable member not interfacing with said chamber, said movable memberhaving a third flow through conduit having a first opening and a secondopening each on a surface of said movable member interfacing with saidchamber, said movable member having a fourth flow through conduit havinga first opening and a second opening each on a surface of said movablemember interfacing with said chamber,
 114. The multiple valve accordingto claim 113, wherein said flow through injection valve furthercomprises: at least one of a (a) first pin isolation valve, (b) secondpin isolation valve, (c) third pin isolation valve, and (d) fourth pinisolation valve; said first pin isolation valve having an internalconduit, said first pin isolation valve disposed within an openingwithin said stationary member interfacing with said chamber so that saidinternal conduit of said first pin isolation valve is movably disposedto be in fluidic communication with said first opening on a first flowthrough conduit of said movable member, said internal conduit of saidfirst pin isolation valve movably disposed to be in fluidiccommunication with said first opening of said third flow throughconduit, said second pin isolation valve having an internal conduit,said second pin isolation valve disposed within an opening within saidstationary member interfacing with said chamber so that said internalconduit of said second pin isolation valve is movably disposed to be influidic communication with said first opening on a second flow throughconduit of said movable member, said internal conduit of said second pinisolation valve movably disposed to be in fluidic communication withsaid second opening of said third flow through conduit, said third pinisolation valve having an internal conduit, said third pin isolationvalve disposed within an opening within said stationary memberinterfacing with said chamber so that said internal conduit of saidthird pin isolation valve is movably disposed to be in fluidiccommunication with said first opening of said fourth flow throughconduit, said internal conduit of said third pin isolation valve movablydisposed to be in fluidic communication with said first opening of saidfirst flow through conduit, said fourth pin isolation valve having aninternal conduit, said fourth pin isolation valve disposed within anopening within said stationary member interfacing with said chamber sothat said internal conduit of said fourth pin isolation valve is movablydisposed to be in fluidic communication with said second opening of saidfourth flow through conduit, said internal conduit of said fourth pinisolation valve movably disposed to be in fluidic communication withsaid first opening of said second flow through conduit.
 115. Themultiple valve according to claim 113, wherein at least one of said flowthrough isolation valve and said flow through injection valve furthercomprises a housing enclosing said stationary member and said movablemember.
 116. The multiple valve according to claim 114, wherein at leastone of said flow through isolation valve and said flow through injectionvalve further comprises a housing enclosing said stationary member andsaid movable member and at least one of said pin isolation valves, saidinternal conduit of said at least one pin isolation valve fluidicallycoupled to a conduit penetrating said housing.
 117. The multiple valveaccording to claim 113, further comprising driving means for driving atleast one of said movable members.
 118. The multiple valve according toclaim 117, wherein said driving means is a linear electric motor. 119.The multiple valve according to claim 113, wherein either of said firstand second pin isolation valves of said flow through injection valve isfluidically coupled to a sample loop of a high pressure liquidchromatography (HPLC) system.
 120. The multiple valve according to claim113, wherein either of said third and fourth pin isolation valves ofsaid flow through isolation valve is fluidically coupled to a pumpsupplying high pressure liquid to a high pressure liquid chromatography(HPLC) system.
 121. The multiple valve according to claim 113, whereineither of said third and fourth pin isolation valves of said flowthrough isolation valve is fluidically coupled to a column discharginghigh pressure liquid from a high pressure liquid chromatography (HPLC)system.
 122. The multiple valve according to claim 115, wherein saidhousing of said at least one of said flow through isolation valve andsaid flow through injection valve is capable of retaining pressuregreater than atmospheric pressure.
 123. The multiple valve according toclaim 116, wherein said housing of said at least one of said flowthrough isolation valve and said flow through injection valve is capableof retaining pressure greater than atmospheric pressure.
 124. Themultiple valve according to claim 113, wherein at least one of saidmovable members is comprised of PEEK (polyetheretherketone) blend. 125.The multiple valve according to claim 113, wherein said interfacingsurface between said stationary member and said movable member of atleast one of said linear injection valve and said linear isolation valveis sealed by at least one lip seal.
 126. The multiple valve according toclaim 114, wherein at least one of said openings of said pin isolationvalves of said linear valves is sealed by a lip seal.
 127. The multiplevalve according to claim 125 wherein said lip seal is self-energizing.128. The multiple valve according to claim 126, wherein said lip seal isself-energizing.
 129. The multiple valve according to claim 113, whereinsaid movable member consists of at least one of (a) metal, (b) polymer,and (c) sapphire.
 130. A method of operating a flow through injectionvalve, the valve comprising: a movable member, the movable member havingfirst and second conduits for interfacing with internal conduits offirst and second pin isolation valves, said first and second conduitsopening to a surface of said movable member; a third conduit enablingfluidic communication between said internal conduits of said first andsecond pin isolation valves; a fourth conduit enabling fluidiccommunication between internal conduits of third and fourth pinisolation valves, said third pin isolation valve providing fluid flow,said fourth pin isolation valve exhausting said fluid flow; (A) whereinthe valve is in an initial position of flow isolation such that saidthird pin isolation valve providing fluid flow is in fluidiccommunication with said fourth pin isolation valve exhausting said fluidflow, said first pin isolation valve is in fluidic communication withsaid first conduit, and said second pin isolation valve is in fluidiccommunication with said second conduit; the method comprising the stepsof: (I) wherein said first pin isolation valve interfaces with saidfirst conduit, (1) moving said first pin isolation valve away from saidfirst conduit; (2) moving said movable member, (3) moving said first pinisolation valve towards said movable member such that said internalconduit within said first pin isolation valve interfaces with said-thirdconduit; and (II) wherein said second pin isolation valve interfaceswith said second conduit, (1) moving said second pin isolation valveaway from said second conduit; (2) moving said movable member, (7)moving said second pin isolation valve towards said movable member suchthat said internal conduit with said second pin isolation valveinterfaces with said third conduit, thereby establishing fluidiccommunication between said first and second pin isolation valves; and(III) wherein said third pin isolation valve interfaces with said fourthconduit, (1) moving said third pin isolation valve away from said fourthconduit; (2) moving said movable member; (3) moving said third pinisolation valve towards said first conduit to establish fluidiccommunication with said internal conduit of said third pin isolationvalve; and (IV) wherein said fourth pin isolation valve interfaces withsaid fourth conduit, (1) moving said fourth pin isolation valve awayfrom said fourth conduit; (2) moving said movable member; (3) movingsaid fourth pin isolation valve towards said second conduit to establishfluidic communication with said internal conduit of said fourth pinisolation valve; and (B) wherein the valve is in an initial position offlow throughput such that at least one of (a) said third pin isolationvalve providing fluid flow interfaces with said first conduit and (b)said fourth pin isolation valve exhausting said fluid flow interfaceswith said second conduit, the method comprising the steps of: (III)wherein said third pin isolation valve interfaces with said firstconduit, (1) moving said third pin isolation valve away from said firstconduit, (2) moving said movable member, and (3) moving said third pinisolation valve towards said movable member such that said internalconduit within said third pin isolation valve interfaces with saidfourth conduit; and (IV) wherein said fourth pin isolation valveinterfaces with said second conduit, (1) moving said fourth pinisolation valve away from said second conduit, (2) moving said movablemember, and (3) moving said fourth pin isolation valve towards saidmovable member such that said internal conduit within said second pinisolation valve interfaces with said first conduit; and (V) wherein saidfirst pin isolation valve interfaces with said third conduit, (1) movingsaid first pin isolation valve away from said third conduit, (2) movingsaid movable member, and (3) moving said first pin isolation valvetowards said movable member such that said internal conduit within saidfirst pin isolation valve interfaces with said first conduit; and (VI)wherein said second pin isolation valve interfaces with said thirdconduit, (1) moving said second pin isolation valve away from said thirdconduit, (2) moving said movable member, and (3) moving said second pinisolation valve towards said movable member such that said internalconduit within said second pin isolation valve interfaces with saidsecond conduit.
 131. A method of operating a multiple valve, themultiple valve comprising a flow through isolation valve, the flowthrough isolation valve comprising: a movable member, the movable memberhaving first and second conduits for interfacing with internal conduitsof first and second pin isolation valves, said conduits opening to asurface of said movable member; first and second blank openings forinterfacing with said internal conduits of said first and second pinisolation valves, (A) wherein the valve is in an initial position offlow isolation such that at least one of (a) said first pin isolationvalve providing fluid flow interfaces with said first blank opening and(b) said second pin isolation valve exhausting said fluid flowinterfaces with said second blank opening, the method comprising thesteps of: (I) wherein said first pin isolation valve interfaces withsaid first blank opening, (1) moving said first pin isolation valve awayfrom said first blank opening, (2) moving said movable member, and (3)moving said first pin isolation valve towards said movable member suchthat said internal conduit within said first pin isolation valveinterfaces with said first conduit opening to a surface of said movablemember; and (II) wherein said second pin isolation valve interfaces withsaid second blank opening, (1) moving said second pin isolation valveaway from said second blank opening, (2) moving said movable member, and(3) moving said second pin isolation valve towards said movable membersuch that said internal conduit within said second pin isolation valveinterfaces with said second conduit opening to a surface of said movablemember, and (B) wherein the valve is in an initial position of flowthroughput such that at least one of (a) said first pin isolation valveproviding fluid flow interfaces with said first conduit and (b) saidsecond pin isolation valve exhausting said fluid flow interfaces withsaid second conduit, the method comprising the steps of: (III) whereinsaid first pin isolation valve interfaces with said first conduit, (1)moving said first pin isolation valve away from said first conduit, (2)moving said movable member, and (3) moving said first pin isolationvalve towards said movable member such that said internal conduit withinsaid first pin isolation valve interfaces with said first blank opening;and (IV) wherein said second pin isolation valve interfaces with saidsecond conduit, (1) moving said second pin isolation valve away fromsaid second conduit, (2) moving said movable member, and (3) moving saidsecond pin isolation valve towards said movable member such that saidinternal conduit within said second pin isolation valve interfaces withsaid second blank opening; and the multiple valve comprising a flowthrough injection valve, the flow through injection valve comprising: amovable member, the movable member having first and second conduits forinterfacing with internal conduits of first and second pin isolationvalves, said first and second conduits opening to a surface of saidmovable member; a third conduit enabling fluidic communication betweensaid internal conduits of said first and second pin isolation valves; afourth conduit enabling fluidic communication between internal conduitsof third and fourth pin isolation valves, said third pin isolation valveproviding fluid flow, said fourth pin isolation valve exhausting saidfluid flow; (A) wherein the valve is in an initial position of flowisolation such that said third pin isolation valve providing fluid flowis in fluidic communication with said fourth pin isolation valveexhausting said fluid flow, said first pin isolation valve is in fluidiccommunication with said first conduit, and said second pin isolationvalve is in fluidic communication with said second conduit; the methodcomprising the steps of: (I) wherein said first pin isolation valveinterfaces with said first conduit, (1) moving said first pin isolationvalve away from said first conduit; (2) moving said movable member, (3)moving said first pin isolation valve towards said movable member suchthat said internal conduit within said first pin isolation valveinterfaces with said third conduit; and (II) wherein said second pinisolation valve interfaces with said second conduit, (1) moving saidsecond pin isolation valve away from said second conduit; (2) movingsaid movable member, (7) moving said second pin isolation valve towardssaid movable member such that said internal conduit with said second pinisolation valve interfaces with said third conduit, thereby establishingfluidic communication between said first and second pin isolationvalves; and (III) wherein said third pin isolation valve interfaces withsaid fourth conduit, (1) moving said third pin isolation valve away fromsaid fourth conduit; (2) moving said movable member; (3) moving saidthird pin isolation valve towards said first conduit to establishfluidic communication with said internal conduit of said third pinisolation valve; and (IV) wherein said fourth pin isolation valveinterfaces with said fourth conduit, (1) moving said fourth pinisolation valve away from said fourth conduit; (2) moving said movablemember; (3) moving said fourth pin isolation valve towards said secondconduit to establish fluidic communication with said internal conduit ofsaid fourth pin isolation valve; and (B) wherein the valve is in aninitial position of flow throughput such that at least one of (a) saidthird pin isolation valve providing fluid flow interfaces with saidfirst conduit and (b) said fourth pin isolation valve exhausting saidfluid flow interfaces with said second conduit, the method comprisingthe steps of: (III) wherein said third pin isolation valve interfaceswith said first conduit, (1) moving said third pin isolation valve awayfrom said first conduit, (2) moving said movable member, and (3) movingsaid third pin isolation valve towards said movable member such thatsaid internal conduit within said third pin isolation valve interfaceswith said fourth conduit; and (IV) wherein said fourth pin isolationvalve interfaces with said second conduit, (1) moving said fourth pinisolation valve away from said second conduit, (2) moving said movablemember, and (3) moving said fourth pin isolation valve towards saidmovable member such that said internal conduit within said second pinisolation valve interfaces with said first conduit; and (V) wherein saidfirst pin isolation valve interfaces with said third conduit, (1) movingsaid first pin isolation valve away from said third conduit, (2) movingsaid movable member, and (3) moving said first pin isolation valvetowards said movable member such that said internal conduit within saidfirst pin isolation valve interfaces with said first conduit; and (VI)wherein said second pin isolation valve interfaces with said thirdconduit, (1) moving said second pin isolation valve away from said thirdconduit, (2) moving said movable member, and (3) moving said second pinisolation valve towards said movable member such that said internalconduit within said second pin isolation valve interfaces with saidsecond conduit.
 132. The method of operating a flow through injectionvalve according to claim 130, wherein said first and second conduitsopening to a surface of said movable member are in fluidic communicationwith a sample loop of a high pressure liquid chromatography (HPLC)system.
 133. The method of operating a flow through injection valveaccording to claim 130, wherein said first and second pin isolationvalves are in fluidic communication with a needle and a syringe of ahigh pressure liquid chromatography (HPLC) system.
 134. The method ofoperating a flow through injection valve according to claim 130, whereinsaid third and fourth pin isolation valves are in fluidic communicationwith a pump and a column of a high pressure liquid chromatography (HPLC)system,
 135. The method of operating a multiple valve according to claim131, wherein said first and second conduits opening to a surface of saidmovable member of said flow through injection valve are in fluidiccommunication with a sample loop of a high pressure liquidchromatography (HPLC) system,
 136. The method of operating a multiplevalve according to claim 131, wherein said first and second pinisolation valves of said flow through injection valve are in fluidiccommunication with a needle and a syringe of a high pressure liquidchromatography (HPLC) system,
 137. The method of operating a multiplevalve according to claim 131, wherein said third and fourth pinisolation valves of said flow through injection valve are in fluidiccommunication with a pump and a column of a high pressure liquidchromatography (HPLC) system,